Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is one of the most destructive pests of several vegetables and fruits worldwide. In spite of various control methods, this pest has still continued to cause significant damage. In this study, the culturable bacterial flora of S. littoralis was determined. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2014) 38: 99-110 © TÜBİTAK doi: Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents 1 2 1 1, Filiz ÖZKAN ÇAKICI , Ali SEVİM , Zihni DEMİRBAĞ , İsmail DEMİR * Department of Biology, Faculty of Science, Karadeniz Technical University, Trabzon, Turkey 2 Department of Environmental Engineering, Faculty of Engineering and Architecture, Ahi Evran University, Kırşehir, Turkey 1 Received: Accepted: Published Online: Printed: Abstract: Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is one of the most destructive pests of several vegetables and fruits worldwide. In spite of various control methods, this pest has still continued to cause significant damage. In this study, the culturable bacterial flora of S. littoralis was determined. New isolates from S. littoralis, as well as 12 different Bacillus isolates belong to 5 species that were previously isolated from different pests, were tested on S. littoralis larvae. In total, 9 bacteria were characterized based on their morphological, biochemical, physiological, and molecular characteristics. The bacterial flora of S. littoralis was determined as Flavobacterium sp. (SL1), Klebsiella pneumonia (SL2), Enterobacter sp. (SL3), Enterobacter sp. (SL4), Klebsiella sp. (SL5), Serratia marcescens (SL6), Pseudomonas aeruginosa (SL7), Acinetobacter baumannii (SL8), and Staphylococcus sp. (SL9). The insecticidal activity tests were performed on the third-instar larvae of S. littoralis. SL1 and SL5 from S. littoralis caused the highest mortalities with 67% and 77%, respectively. Among previously isolated Bacillus isolates, Bacillus thuringiensis subsp. kurstaki (MnD) and B. thuringiensis subsp. kurstaki (BnBt) were found to be the most .
