Iron (Fe) deficiency is one of the common problems in strawberry (Fragaria × ananassa Duch.) growing regions. In vitro screening enables researchers to evaluate genotype performance under several concentrations of Fe in a relatively fast and economical way. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2014) 38: 169-179 © TÜBİTAK doi: Research Article In vitro screening of octoploid Fragaria chiloensis and Fragaria virginiana genotypes against iron deficiency 1, 2 2 1 3,4 Ayfer ALKAN TORUN *, Yıldız AKA KAÇAR , Belgin BİÇEN , Nazife ERDEM , Sedat SERÇE Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Çukurova University, Adana, Turkey 2 Department of Horticulture, Faculty of Agriculture, Çukurova University, Adana, Turkey 3 Department of Horticulture, Faculty of Agriculture, Mustafa Kemal University, Adana, Turkey 4 Department of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technologies, Niğde University, Niğde, Turkey 1 Received: Accepted: Published Online: Printed: Abstract: Iron (Fe) deficiency is one of the common problems in strawberry (Fragaria × ananassa Duch.) growing regions. In vitro screening enables researchers to evaluate genotype performance under several concentrations of Fe in a relatively fast and economical way. Therefore, we evaluated the responses of several genotypes belonging to octoploid Fragaria chiloensis (L.) Mill. and Fragaria virginiana Mill. (the progenitors of cultivated strawberry) against Fe treatments under in vitro conditions. In the first experiment, HM 1, Pigeon Point, CFRA1267 (F. chiloensis subsp. pacifica), and CA1541 (F. chiloensis subsp. chiloensis) were tested with various Fe concentrations (0%, 10%, 15%, 25%, 50%, 65%, 80%, and 100%, where 100% = mg L–1 ). Different Fe concentration treatments resulted in differences in plant height, fresh and dry weight, as well as macro- and microelement concentrations among the tested genotypes. In the second experiment, 16 octoploid Fragaria genotypes were tested with 4 Fe concentration treatments (0%, 10%, 50%, and 100%, where 100% = .