In this study, DNA sequencing and single-stranded conformation polymorphism (SSCP) analyses were performed to determine the allelic diversity of genes associated with tolerance to iron chlorosis in citrus. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2014) 38: 340-347 © TÜBİTAK doi: Genetic relationships of some Citrus genotypes based on the candidate iron chlorosis genes 1, 1 2 1 1 3 Yıldız AKA KAÇAR *, Özhan ŞİMŞEK , Dicle DÖNMEZ , Melda BONCUK , Turgut YEŞİLOĞLU , Patrick OLLITRAULT 1 Department of Horticulture, Faculty of Agriculture, Çukurova University, Adana, Turkey 2 Department of Biotechnology, Institute of Basic and Applied Science, Çukurova University, Adana, Turkey 3 CIRAD-BIOS, UMR AGAP, Montpellier, France Received: Accepted: Published Online: Printed: Abstract: Iron is one of the most important elements in plant mineral nutrition. Fe deficiency is a critical abiotic stress factor for Mediterranean citriculture; the development of marker-assisted selection for this trait would greatly enhance rootstock breeding. In this study, DNA sequencing and single-stranded conformation polymorphism (SSCP) analyses were performed to determine the allelic diversity of genes associated with tolerance to iron chlorosis in citrus. Two candidate iron chlorosis tolerance genes were selected from existing Citrus EST databases and Arabidopsis thaliana genome databases. Ferritin-3 chloroplast precursor and putative membrane transporter candidate gene sequences were used to define primers in conserved regions. Six citrus genotypes from the basic taxon of Citrus were used to identify polymorphic regions in the genes. Direct sequencing of the amplified DNA fragments from the candidate genes was performed, and single nucleotide polymorphisms (SNPs) and insertions/deletions (indels) were identified after sequence alignment. Based on the DNA sequencing analysis, a total of 6840 nucleotides of DNA were sequenced to identify SNPs and indels. In total, 263 SNPs and 15 indels were identified for both genes. We detected SNPs and
