Nigella sativa L. has industrial, cosmetic, and pharmaceutical uses but has not been adequately characterized in Pakistan. Th is investigation was carried out to explore genotype specific fingerprinting of 32 N. sativa L. genotypes based on randomly amplified polymorphic DNA markers. | M. S. IQBAL, S. NADEEM, S. MEHBOOB, A. GHAFOOR, M. I. RAJOKA, A. S. QURESHI, B. NIAZ Research Article Turk J Agric For 35 (2011) 569-578 © TÜBİTAK doi: Exploration of genotype specific fingerprinting of Nigella sativa L. using RAPD markers Muhammad Sajjad IQBAL1,2,5,*, Shahid NADEEM2, Shahid MEHBOOB2, Abdul GHAFOOR3, Muhammad Ibrahim RAJOKA2, Afsari Sharif QURESHI1, Bushra NIAZ4 1 Genetics Laboratory, Department of Biochemistry, Quaid-I-Azam University, Islamabad, 46000 - PAKISTAN 2 Department of Bioinformatics, GC University, Allama Iqbal Road, 38000, Faisalabad - PAKISTAN 3 Institute of Agri-Biotechnology & Genetic Resources, National Agricultural Research Center, Islamabad, 45500 - PAKISTAN 4 Department of Post-Harvest, Ayub Agriculture Research Institute, Faisalabad - PAKISTAN 5 Department of Genetics, Hazara University, Mansehra, 21300 - PAKISTAN Received: Abstract: Nigella sativa L. has industrial, cosmetic, and pharmaceutical uses but has not been adequately characterized in Pakistan. This investigation was carried out to explore genotype specific fingerprinting of 32 N. sativa L. genotypes based on randomly amplified polymorphic DNA markers. From 58 random primers used, 15 primers generated 249 reproducible and scorable amplification products across all the genotypes, out of which 164 (66%) fragments were polymorphic revealing a high level of polymorphism among these genotypes. The proportion of common bands was low (34%). The size of the amplification products on agarose gels ranged between and kb. In 13 genotypes, 27 bands of different masses (kilobases) were recorded and were considered specific to those genotypes. These specific/amplified PCR products can be used as molecular markers for identification of germplasm and resource protection of Nigella sativa L. genotypes. Specific bands were observed for individual primers that could resolve genetic diversity among several genotypes (PK-020545, PK-020567,
