The objective of this study was to discriminate 11 maize hybrids with one parent in common using ultrathinlayer isoelectric focusing (UTLIEF) of seed salt-soluble proteins in NaCl solution in the laboratory. | . DOU, M. YAN, . XU, K. HUSSAIN, . LIU, F. LIN Research Article Turk J Agric For 36 (2012) 267-273 © TÜBİTAK doi: Identification and purity testing of maize hybrids with one parent in common by ultrathin-layer isoelectric focusing of seed salt-soluble proteins Xiao-Yue DOU1, Min YAN2, Yu-Feng XU1, Khalid HUSSAIN3, Yuan-Zhi LIU 4, Feng LIN 1,* 1 Biotechnology and Bioscience College, Shenyang Agricultural University, Shenyang 11016 - CHINA 2 College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109 - CHINA 3 Department of Botany, University of Gujrat (UOG), Gujrat 50700 - PAKISTAN 4 Maize Science Institute, Shenyang Academy of Agricultural Sciences, Shenyang 110034 - CHINA Received: Abstract: The objective of this study was to discriminate 11 maize hybrids with one parent in common using ultrathinlayer isoelectric focusing (UTLIEF) of seed salt-soluble proteins in NaCl solution in the laboratory. Thirtytwo protein bands were found in the pH range of , of which were polymorphic. Six hybrids with the same male parent and 5 hybrids with the same female parent were differentiated by their patterns and numbers of polymorphic protein bands. The result showed that UTLIEF has the characteristics of high resolution to discriminate close genetic relationships. Meanwhile, the genetic purity of 11 hybrids were tested, and the purities for different hybrids were as follows: Shenyu10, 96%; Shenyu13, ; Shenyu16, 98%; Shenyu17, ; Shenyu18, 96%; Shenyu23, ; Shenyu21, ; Shenyu22, ; Shenyu29, ; Shenyu30, 100%; and Shenyu31, 98%. Key words: Isoelectric focusing, maize, purity testing, salt-soluble proteins, ultrathin-layer Introduction Maize (Zea mays L.) is one of the most extensively cultivated cereal crops on earth, and almost every country in the world cultivates maize commercially for a variety of uses. Maize has good heterosis potential for .
