Three newly selected superior banana types were used to study the effects of different cytokinins and auxins on shoot multiplication and rooting. Benzylaminopurine [(BAP) (5, 10, 20 and 30 µM)] and thidiazuron [(TDZ) (, 1, 2 and 3 µM)] were tested alone and with 1 µM indoleacetic acid (IAA) for the propagation stage. | Turk J Agric For 28 (2004) 355-361 © TÜB‹TAK In Vitro Propagation of Some New Banana Types (Musa spp.) Hamide GÜBBÜK*, Mustafa PEKMEZC‹ Department of Horticulture, Faculty of Agriculture, Akdeniz University 07059 Antalya - TURKEY Received: Abstract: Three newly selected superior banana types were used to study the effects of different cytokinins and auxins on shoot multiplication and rooting. Benzylaminopurine [(BAP) (5, 10, 20 and 30 µM)] and thidiazuron [(TDZ) (, 1, 2 and 3 µM)] were tested alone and with 1 µM indoleacetic acid (IAA) for the propagation stage. We compared basal Murashige and Skoog (MS) -1 medium, active charcoal (5 g l ), indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) for rooting. Shoot proliferation and elongation were significantly greater with TDZ than with BAP all 3 types. Furthermore, each cytokinin with IAA increased shoot proliferation and elongation more than their use alone. BAP below 20 µM or TDZ below 1 µM did not increase shoot proliferation, and BAP over 20 µM and TDZ over 2 µM suppressed shoot elongation. Charcoal alone was better for rooting than auxin treatments or MS medium alone. In conclusion, supplementation of 2 µM TDZ, and 1 µM IAA or 20 µM BAP and 1 µM IAA on MS medium, followed 5 g l-1 charcoal at the rooting stage were the best combinations for the in vitro propagation of banana types. Key Words: Banana, Musa spp., tissue culture, thidiazuron, active charcoal Baz› Yeni Muz Tiplerinin (Musa spp.) Doku Kültürü ile Ço¤alt›lmas› Özet: Bu çal›flmada deneme materyali olarak, yeni selekte edilmifl 3 superior muz tipi kullan›lm›fl ve muz tiplerinin ço¤alt›lmas› ve köklendirilmesi üzerine farkl› sitokinin ve oksin tiplerinin etkileri araflt›r›lm›flt›r. Bu amaçla, ço¤altma aflamas›nda de¤iflik benzylaminopurine [(BAP) (5, 10, 20 and 30 µM)] ve thidiazuron [(TDZ) (, 1, 2 and 3 µM)] konsantrasyonlar›n›n ba¤›ms›z ve 1 µM indoleacetic asit (IAA) ile olan kombinasyonlar› test edilmifltir. Ayr›ca .
