The prevalence of apple chlorotic leaf spot virus (ACLSV) was investigated in all the main stone fruit growing areas of Turkey except for East Anatolia. Three hundred and sixty-nine plant samples showing virus related symptoms were collected from sweet and sour cherry, peach, nectarine, apricot and plum trees. | Turk J Agric For 29 (2005) 251-257 © TÜB‹TAK Apple Chlorotic Leaf Spot Virus (ACLSV) Status in Turkey and Sensitive Detection Using Advanced Techniques Çi¤dem ULUBAfi* Mustafa Kemal University, Plant Protection Department 31034 Antakya, Hatay - TURKEY Filiz ERTUNÇ Ankara University, Plant Protection Department 06100 D›flkap›, Ankara - TURKEY Received: Abstract: The prevalence of apple chlorotic leaf spot virus (ACLSV) was investigated in all the main stone fruit growing areas of Turkey except for East Anatolia. Three hundred and sixty-nine plant samples showing virus related symptoms were collected from sweet and sour cherry, peach, nectarine, apricot and plum trees. All samples were tested using DAS-ELISA and reverse transcriptionpolymerase chain reaction (RT-PCR). Thirteen samples were detected as infected with ACLSV using ELISA while RT-PCR revealed 51 positive samples. The highest ACLSV incidence was in Bursa (64%), followed by Yalova (), Burdur (), Amasya (), Afyon (), Isparta (), Çanakkale () and ‹zmir (). Among the collected stone fruit species, nectarine was the most infected species, followed by apricot, peach, sour cherry, plum and sweet cherry. Some ACLSV isolates were used for the adoption of an immuno capture (IC) RT-PCR system. ACLSV infected plant sap diluted up to 1:10 was detected by ICRT-PCR. The successful application of RT-PCR and IC-RT-PCR for the accurate and sensitive detection of ACLSV was reported in stone fruit trees in Turkey. Key Words: Apple chlorotic leaf spot virus, Reverse transcription-polymerase chain reaction, Turkey Elma Klorotik Leke Virüsü (ACLSV)’nün Türkiye’deki Durumu ve ‹leri Tekniklerle Hassas Teflhisi Özet: Sert çekirdekli meyve yetifltiricili¤inin Türkiye’de, Do¤u Anadolu Bölgesi d›fl›nda, yo¤un oldu¤u bölgelerde Elma klorotik yaprak lekesi virüsü (ACLSV)’nün yayg›nl›¤› araflt›r›lm›flt›r. Toplanan tüm virüs simptomu gösteren kiraz, fleftali, nektarin, kay›s› ve .
