Intra- and interspecific polymorphisms among fungal pathogens that cause wilt and root rot on chickpea were investigated by using 30 RAPD (random amplified polymorphic DNA) and 20 ISSR (inter-simple sequence repeats) primers. UPGMA (unweighted pair group method with arithmetic average) cluster analysis of RAPD and ISSR datasets using Dice’s coefficient differentiated all fungal isolates from each other and revealed considerable genetic variability between the isolates. | Turk J Agric For 33 (2009) 1-10 © TÜBİTAK doi: Genetic Diversity of Wilt and Root Rot Pathogens of Chickpea, as Assessed by RAPD and ISSR Harun BAYRAKTAR*, F. Sara DOLAR Department of Plant Protection, Faculty of Agriculture, University of Ankara, 06110 Ankara - TURKEY Received: Abstract: Intra- and interspecific polymorphisms among fungal pathogens that cause wilt and root rot on chickpea were investigated by using 30 RAPD (random amplified polymorphic DNA) and 20 ISSR (inter-simple sequence repeats) primers. UPGMA (unweighted pair group method with arithmetic average) cluster analysis of RAPD and ISSR datasets using Dice’s coefficient differentiated all fungal isolates from each other and revealed considerable genetic variability between the isolates. The isolates that were not easily separable using morphological characteristics were classified again according to their branching in dendrograms. A partial correlation was observed between cluster analysis and geographic origin of Fusarium solani isolates. Additionally, high cophenetic correlation values for RAPD and ISSR data, using the MXCOPH algorithm, were observed ( and , respectively). The results show that both analyses were suitable for the evaluation of genetic polymorphisms among fungal populations. Key Words: Chickpea, root rot, genetic diversity, RAPD, ISSR Nohut Solgunluk ve Kök Çürüklüğü Patojenleri Arasındaki Genetik Farklılığın RAPD ve ISSR ile İncelenmesi Özet: Nohutta solgunluk ve kök çürüklüğüne sebep olan fungal patojenler arasındaki intra ve interspesifik polimorfizimler 30 RAPD (Random amplified polymorphic DNA) ve 20 ISSR (Inter-simple sequence repeats) primeri kullanılarak incelenmiştir. Elde edilen RAPD ve ISSR verilerinin Dice’ ın benzerlik katsayısı kullanılarak yapılan UPGMA (Unweighted Pairgroup Method with Arithmetic Average) cluster analizi sonucunda tüm fungus izolatları birbirinden ayrı olarak gruplandırılmış ve aralarında önemli genetik
