DNA microarrays, being high-density and high-throughput, allow quantitative analyses of thousands of genes and their expression patterns in parallel. In this study, Barley1 GeneChip was used to investigate transcriptome changes associated with boron (B) toxicity in a sensitive barley cultivar (Hordeum vulgare L. cv. Hamidiye). | Turk J Agric For 33 (2009) 191-202 © TÜBİTAK doi: Microarray Analysis of Late Response to Boron Toxicity in Barley (Hordeum vulgare L.) Leaves Mehmet Tufan ÖZ1, Remziye YILMAZ2, Füsun EYİDOĞAN3, Leo de GRAAFF4, Meral YÜCEL1,2, Hüseyin Avni ÖKTEM1,* 1 Middle East Technical University, Department of Biological Sciences, Plant Biotechnology R&D Labs, Ankara - TURKEY 2 Middle East Technical University, Central Laboratory, Molecular Biology Biotechnology R&D Center, Ankara - TURKEY 3 4 Başkent University, Faculty of Education, Ankara - TURKEY Wageningen University, Laboratory of Microbiology, Fungal Genomics Group, Wageningen – NETHERLANDS Received: Abstract: DNA microarrays, being high-density and high-throughput, allow quantitative analyses of thousands of genes and their expression patterns in parallel. In this study, Barley1 GeneChip was used to investigate transcriptome changes associated with boron (B) toxicity in a sensitive barley cultivar (Hordeum vulgare L. cv. Hamidiye). Eight-day-old aseptically grown seedlings were subjected to 5 or 10 mM boric acid (B(OH)3) treatments for 5 days and expression profiles were determined with DNA microarrays using total RNA from leaf tissues. Among the 22,840 transcripts – each represented with a probe set on the GeneChip – 19,424 probe sets th showed intensity values greater than 20 percentile in at least one of the hybridizations. Compared to control (10 μM B(OH)3), 5 mM B(OH)3 treatment resulted in differential expression of 168 genes at least by twofold. Moreover, 10 mM B(OH)3 treatment resulted in at least twofold induction or reduction in expression of 312 transcripts. Among these genes, 37 and 61 exhibited significantly (P #" # # # #" ! ! ! !" # # #" # # #" $ ' !" ! " +!= ! > " Figure 1. Normalization and .
