Mating type groups of ascochyta rabiei (Teleomorph: Didymella rabiei), the causal agent of chickpea blight in Central Anatolia

The distribution of mating types of Ascochyta rabiei (teleomorph: Didymella rabiei) was studied on isolates collected from chickpea growing areas of Central Anatolia. Mating type assessments of 45 isolates from 6 different provinces were conducted under laboratory conditions. | Turk J Agric For 31 (2007) 41-46 © TÜB‹TAK Mating Type Groups of Ascochyta rabiei (Teleomorph: Didymella rabiei), the Causal Agent of Chickpea Blight in Central Anatolia Harun BAYRAKTAR*, F. Sara DOLAR, Salih MADEN Department of Plant Protection, Faculty of Agriculture, University of Ankara, 06110, Ankara - TURKEY Received: Abstract: The distribution of mating types of Ascochyta rabiei (teleomorph: Didymella rabiei) was studied on isolates collected from chickpea growing areas of Central Anatolia. Mating type assessments of 45 isolates from 6 different provinces were conducted under laboratory conditions. After keeping for 5-6 weeks, the pseudothecial development and ascospore production were observed on chickpea stem pieces that were incubated in 8 ± 1 °C and 85% relative humidity. Both mating types were found in all the provinces, except for Kayseri and Sivas. The majority of the isolates belonged to Mat 1-1 () and the others () to MAT 1-2. Additionally, the mating types of A. rabiei were differentiated from each other using the multiplex PCR method with the primers Com1, Tail 5, and SP21. MAT 1-1 isolates were amplified to approximately a 460 bp fragment and MAT 1-2 isolates to a 700 bp fragment, in accordance with the discrimination of mating type groups with conventional methods. Key Words: Chickpea, Ascochyta rabiei, multiplex PCR, mating type Nohutlarda Yan›kl›k Etmeni Ascochyta rabiei’nin (Teleomorph: Didymella rabiei) Orta Anadolu Bölgesindeki Mating Type Gruplar› Özet: Orta Anadolu bölgesindeki 6 farkl› nohut ekim alan›ndan toplanan 45 Ascochyta rabiei (teleomorph: Didymella rabiei) izolat›n›n mating type gruplar›n›n da¤›l›m› laboratuar koflullar›nda araflt›r›lm›flt›r. Nohut gövde parçalar› üzerinde pseudothecium geliflimi ve ascospor üretimi 8 ± 1 °C s›cakl›k ve % 85 nispi nem içeren koflullarda 5-6 haftal›k inkübasyon periyodundan sonra gözlenmifltir. Kayseri ve Sivas illeri hariç di¤er tüm illerde her iki mating type grubu da .