Transfer of a β-Glucuronidase reporter gene to sugarbeet (Beta vulgaris L.) via microprojectile bombardment

For the transfer of a β-Glucuronidase (GUS) reporter gene to sugarbeet (Beta vulgaris L.) callus and leaf explants by microprojectile bombardment, various rupture disk pressures and sample plate distances were tested. | Turk J Agric For 24 (2000) 487–490 © TÜBİTAK Transfer of a β-Glucuronidase Reporter Gene to Sugarbeet (Beta vulgaris L.) Via Microprojectile Bombardment* Sertaç ÖNDE Department of Biological Sciences, Faculty of Arts and Sciences, Middle East Technical University, 06531 Ankara-TURKEY Melahat BİRSİN, Mustafa YILDIZ, Cengiz SANCAK, Murat ÖZGEN Department of Field Crops, Faculty of Agriculture, Ankara University, 06110 Dışkapı, Ankara-TURKEY Received: Abstract: For the transfer of a β-Glucuronidase (GUS) reporter gene to sugarbeet (Beta vulgaris L.) callus and leaf explants by microprojectile bombardment, various rupture disk pressures and sample plate distances were tested. By coating the DNA construct over the gold particles, bombardments were carried out by the DuPont PDS-1000/He system and the results clearly indicated the superiority of leaf explants over the callus structures as targets. The sample plate distances affected the distribution pattern of the particles and the cells expressing the GUS reporter gene were noted to be aggregated in short distances whereas longer distance shots yielded better distribution of transformed cells. For both leaf explants and callus structures the rupture disk pressure of 1350 psi was observed to yield the highest gene expression results and the sample plate distance of 12 cm for the leaf explants and 9 cm for the callus were found to increase the penetration success of the gold particles. Şekerpancarına (Beta vulgaris L.) Partikül Bombardımanı Yöntemiyle β-Glukuronidaz Raportör Geni Transferi Özet: Şekerpancarı (Beta vulgaris L.) yaprak ve kallus eksplantlarına partikül bombardımanı yöntemiyle β-glukuronidaz (GUS) raportör geninin aktarılmasında değişik basınç ve mesafeler denendi. Altın partiküllerine DNA yapısının bağlanmasıyla ve DuPont PDS-1000/He sisteminden yararlanılarak yapılan atışlar sonucunda yaprakların, kalluslara oranla daha iyi bir hedef doku oldukları belirlendi. Yakın .