Desiccation of somatic embryos under different air humidities conditioned by saturated salt solutions for different durations relationships between desiccation and germination or root formation in the dark and effects of desiccation and gibberellic acid on germinating of 'Su-2' walnut somatic embryonic embryo line were investigated. | Turk J Agric For 24 (2000) 491–498 © TÜBİTAK Dessiccation Using Saturated Salt Solutions and Improvement Germination Rate of Walnut (Juglans regia L.) Somatic Embryos Hatice DUMANOĞLU Univ. of Ankara, Fac. of Agriculture, Department of Horticulture, 06110 Ankara-TURKEY Received: Abstract: Desiccation of somatic embryos under different air humidities conditioned by saturated salt solutions for different durations; relationships between desiccation and germination or root formation in the dark; and effects of desiccation and gibberellic acid on germinating of 'Su-2' walnut somatic embryonic embryo line were investigated. Survival of embryos was low (about 10%) or zero when loss of fresh weight was about 90% and dry matter was about 70%. The highest germinating percentages () were obtained with about of loss of fresh weight and of dry matter of desiccated embryos in covered plates over saturated for 4 days, Mg(NO3) for 5 days or NaCl for 5-6 days, and with uncovered plates over for 6-7 days or NH3SO4 for 6-8 days. The highest rooting percentage () in the dark were obtained with of loss of fresh weight and of dry matter of desiccated embryos in covered plates over saturated for 4 days. To improve germination, both desiccation and GA3 treatments were more effective than only desiccation or GA3 treatments on germination. Germinating was best () with desiccation followed by incubation in the dark for 15 days prior to being transferred to a medium containing 9 mg/l GA3 in the light. Ceviz (Juglans regia L.) Somatik Embriyolarının Doymuş Tuz Solusyonları ile Kurutulması ve Embriyolarda Çimlenme Oranının Artırılması Özet: Cevizin (Juglans regia L.) somatik embriyonik embriyo hattı ‘Su-2’da, doymuş tuz solusyonları ile oluşturulan farklı hava nemi koşullarında değişik sürelerde somatik embriyonların kurutulması; kurutma ile karanlık koşullarda çimlenme ya da kök .
