In this research, partial nucleotide sequences of the axi 1 gene, which is related to auxin perception and transduction, isolated from Vicia sativa using cDNA library screening were investigated. Four V. sativa cDNA clones representing homologous of the tobacco axi 1 (auxin independent) cDNA clone were isolated and characterized. | Turk J Agric For 25 (2001) 175-180 © TÜB‹TAK Isolation and Characterization of Sequences Homologous to the Tobacco Clone Axi 1 (auxin independent) from a Vicia sativa Nodule cDNA Library Yeflim YALÇIN-MEND‹, Selim ÇET‹NER Çukurova University, Agriculture Faculty, Horticulture Department, Balcal›, Adana - TURKEY Ton BISSELING Molecular Biology Department, Wageningen Agricultural University, NETHERLANDS Received: Abstract: In this research, partial nucleotide sequences of the axi 1 gene, which is related to auxin perception and transduction, isolated from Vicia sativa using cDNA library screening were investigated. Four V. sativa cDNA clones representing homologous of the tobacco axi 1 (auxin independent) cDNA clone were isolated and characterized. With sequence analysis two different clones were observed, called 10/3a and 16/3b. Sequences about 500 bp from the 5' end and 450 bp from the 3' end were determined from the 10/3a clone. The sequences from the 5' end of cDNA clone 10/3a shared about 58 % similarity at the nucleotide level and 61% identity at the polypeptide level with the sequences of the Nicotiana tabacum axi 1 cDNA clone. At the 3' end of 10/3a, 63 % similarity at the nucleotide level was observed with the clone. In 16/3b about 500 bp was sequenced from the 5' end. There was 64 % nucleotide sequence similarity and 61 % polypeptide sequence identity. Northern blot analysis showed that the size of the mRNA was about 2 kb, like that of the axi 1 isolated from tobacco. It was shown that homolog sequences of axi 1 were expressed in flowers, leaves, stems, roots and nodules of the V. sativa plants. Key Words: axi 1, auxin, gene isolation, Vicia sativa Fi¤ (Vicia sativa) Bitkisinin Nodül cDNA Kütüphanesinden, Tütün Axi 1 (Oksinden Ba¤›ms›z) Klonunun Homolog Dizinlerinin ‹zole Edilmesi ve Karakterizasyonu Özet: Bu araflt›rmada, fi¤ (Vicia sativa) bitkisinin cDNA kütüpanesinden, oksin al›m› ve kullan›m›ndan sorumlu olan axi 1 geninin .
