Fragments containing the internal transcribed spacer (ITS) ribosomal rDNA were amplified from Meloidogyne incognita, M. javanica, M. arenaria and M. hapla by polymerase chain reaction (PCR). | Turk J Agric For 26 (2002) 337-341 © TÜB‹TAK Identification of Root-Knot Nematodes in the Mediterranean Region of Turkey by Using rDNA and mtDNA Markers Zübeyir DEVRAN Citrus and Greenhouse Crops Research Institute, Antalya - TURKEY U¤ur GÖZEL, M. Ali SÖ⁄ÜT, fienol YILDIZ, ‹. Halil ELEKÇ‹O⁄LU Çukurova University, Agriculture Faculty, Plant Protection Department, Adana - TURKEY Received: Abstract: Fragments containing the internal transcribed spacer (ITS) ribosomal rDNA were amplified from Meloidogyne incognita, M. javanica, M. arenaria and M. hapla by polymerase chain reaction (PCR). Only digestion of the ITS region with the enzyme RsaI significantly distinguished M. hapla from other species, while other enzymes, EcoRI and BamHI, were not effective in distinguishing these nematode species. PCR was also done with mitocondrial DNA (mtDNA) markers for species discrimination. The obtained amplification product of 600 bp was digested with HinfI restriction endonuclease allowing discrimination among the four root-knot species. mtDNA was found to be a useful tool for discriminating between the important Meloidogyne species in this study. Key Words: Root-nematodes, rDNA, mtDNA, diagnostics Türkiye’nin Akdeniz Bölgesindeki Kök Ur Nematodlar›n›n rDNA ve mtDNA Markerleri ile Tan›mlanmas› Özet: Meloidogyne incognita, M. javanica, M. arenaria ve M. hapla’n›n rDNA’s›n›n ITS bölgesini içeren fragmentler PCR ile ço¤alt›lm›flt›r. ITS bölgesinin RsaI enzimi ile kesimi sonucu M. hapla di¤er türlerden ayr›lm›flt›r. EcoRI ve BamHI enzimlerinin bu türleri ay›rmada etkisiz oldu¤u belirlenmifltir. Bu nedenle di¤er türleri ay›rmada mtDNA markerleri kullan›lm›flt›r. PCR sonucu elde edilen 600 bp’l›k fragment HinfI restriction enzimi ile kesilerek türler birbirlerinden ayr›lm›flt›r. Bu çal›flmada mtDNA’n›n önemli Meloidogyne türlerini ay›rmada faydal› bir araç olabilece¤i bulunmufltur. Anahtar Sözcükler: Kök ur nematodlar›, rDNA, mtDNA, teflhis Introduction Turkey has a .
