The present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. | Turkish Journal of Biology Turk J Biol (2016) 40: 1227-1234 © TÜBİTAK doi: Research Article Plantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species 1 1 1 Carolina Mariane MOREIRA , Helena Botelho de ANDRADE , Suzan Kelly Vilela BERTOLUCCI , 2 3 1, Osmar Alves LAMEIRA , Aliyu MOHAMMED , José Eduardo Brasil Pereira PINTO * 1 Plant Tissue Culture and Medicinal Plants Laboratory, Department of Agriculture, Federal University of Lavras (UFLA), Lavras, Brazil 2 Genetic Resources and Biotechnology, Embrapa Amazônia Oriental, Belem, Brazil 3 Department of Biology, Faculty of Arts and Sciences, Abant İzzet Baysal University, Bolu, Turkey Received: Accepted/Published Online: Final Version: Abstract: The present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction ( and , respectively). Among the explant types tested, only leaf base segments induced calli; the highest frequency occurred via culture treatment containing 4-amino-3,5,6trichloropicolinic acid (picloram; ), 2,4-D (40%), or TDZ (). However, only calli generated in treatments containing TDZ or N6-isopentenyladenine (2ip) were able to develop shoots (maximum and , respectively). A combination of TDZ and 2,4-D improved callus induction () but did not increase shooting response. However, both callusing and shooting .
