Rosa heckeliana, as one of the wild-growing species of the family Rosacea, has a wide use in the folk medicine, scientifically there is very little known about it. Our objective was to examine the antioxidative and antiproliferative properties of Rosa heckeliana root extract and its phenolics. | Turkish Journal of Biology Turk J Biol (2017) 41: 195-212 © TÜBİTAK doi: Research Article Wild-growing Rosa heckeliana Tratt.: phenolic constituents with cytotoxic and antioxidative properties 1, 2 Nursen ÇORUH *, Nizamettin ÖZDOĞAN Department of Chemistry, Faculty of Arts and Science, Middle East Technical University, Ankara, Turkey 2 Department of Environmental Engineering, Faculty of Engineering, Bülent Ecevit University, Zonguldak, Turkey 1 Received: Accepted/Published Online: Final Version: Abstract: Rosa heckeliana, as one of the wild-growing species of the family Rosacea, has a wide use in the folk medicine; however, scientifically there is very little known about it. Our objective was to examine the antioxidative and antiproliferative properties of Rosa heckeliana root extract and its phenolics. The phenolic constituents, namely catechin, caffeic acid, and ellagic acid, were obtained by the methods of extraction, fractionation, and purification through column chromatography. The DPPH and ABTS radical scavenging assays and total flavonoid content analysis were employed as the current antioxidant methods over the crude extract and fractionated parts. Among the extracts/fractions, the ethyl acetate fraction exhibited the highest amount of flavonoid content ( ± µg CE/ mg of extract). The ethyl acetate fraction also displayed high antioxidative properties for DPPH (EC50 value: ± µg/mL) and ABTS scavenging capacities (586 ± µM trolox as TEAC value). Additionally, the antiproliferative properties of the crude extract and the ethyl acetate fraction and its isolated constituents were evaluated for two breast cancer cell lines (MCF-7 and MDA-MB-231) using the XTT method. Acetate fraction over the cells of MCF-7 and MDA-MB-231 resulted in ED50 values of ± µg/mL and ± µg/mL, respectively. The isolated phenolic constituents
