Teucrium species have been used in traditional medicine for treatment of different diseases. The aim of this study was to investigate polyphenolic contents by high-performance liquid chromatography (HPLC), and the genotoxic effect of methanolic extracts of Teucrium polium and Teucrium scordium using the cytokinesis-block micronucleus (CBMN) assay on human peripheral blood lymphocytes (PBLs) from healthy donors. | Turkish Journal of Biology Turk J Biol (2018) 42: 152-162 © TÜBİTAK doi: Research Article Polyphenolic contents of Teucrium polium L. and Teucrium scordium L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes 1,2 1 1 Olivera MILOŠEVIĆ-DJORDJEVIĆ , Marina RADOVIĆ JAKOVLJEVIĆ , Aleksandra MARKOVIĆ , 1 3 4 1, Milan STANKOVIĆ , Andrija ĆIRIĆ , Dragoslav MARINKOVIĆ , Darko GRUJIČIĆ * 1 Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Kragujevac, Serbia 2 Department of Genetics, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia 3 Department of Chemistry, Faculty of Science, University of Kragujevac, Kragujevac, Serbia 4 Serbian Academy of Sciences and Arts, Belgrade, Serbia Received: Accepted/Published Online: Final Version: Abstract: Teucrium species have been used in traditional medicine for treatment of different diseases. The aim of this study was to investigate polyphenolic contents by high-performance liquid chromatography (HPLC), and the genotoxic effect of methanolic extracts of Teucrium polium and Teucrium scordium using the cytokinesis-block micronucleus (CBMN) assay on human peripheral blood lymphocytes (PBLs) from healthy donors. The HPLC analysis showed that extracts consist of phenolic acid (gallic, vanillic, caffeic, chlorogenic, p-coumaric, sinapic) and flavonoids (catechin, rutin, myricetin, luteolin, quercetin and apigenin). Cultures were treated with extracts of both plants separately and in combinations with mitomycin C (MMC). In separate treatments, both herbal extracts significantly induced micronucleus (MN) frequency only at the highest concentrations. All concentrations of T. scordium, except the lowest, and all concentrations of T. polium extracts in combined treatment with MMC significantly reduced the frequency
