In the present study, differentiation potential of human adipose-derived stem cells (ASCs) to cardiomyocytes has been investigated by growing them on hyaluronic acid/gelatin (HA/G) plasma gels and coverslips and supplementing the growth medium with chemical modifiers (activin-a, BMP-4, insulin, valproic acid, and 5-azacytidine) in various combinations. | Turkish Journal of Biology Turk J Biol (2016) 40: 369-379 © TÜBİTAK doi: Research Article Cardiomyogenic differentiation potential of human lipoaspirate-derived stem cells on hyaluronic acid/gelatin plasma gels 1 2 3 1 3,4, Esra GÖV , Halime KENAR , Zehra Seda HALBUTOĞULLARI , Kazım Yalçın ARĞA , Erdal KARAÖZ * 1 Department of Bioengineering, Faculty of Engineering, Marmara University, Göztepe, İstanbul, Turkey 2 Experimental and Clinical Research Center, Kocaeli University, Kocaeli, Turkey 3 Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli University, Kocaeli, Turkey 4 Center for Regenerative Medicine and Stem Cell Research and Manufacturing (LivMedCell), Liv Hospital, İstanbul, Turkey Received: Accepted/Published Online: Final Version: Abstract: Cardiomyogenic differentiation from mesenchymal stem cells has emerged as a novel approach for repair of damaged myocardium. Cell transplantation through direct cell injection is not an optimal method due to the lack of cell–extracellular matrix interactions. In the present study, differentiation potential of human adipose-derived stem cells (ASCs) to cardiomyocytes has been investigated by growing them on hyaluronic acid/gelatin (HA/G) plasma gels and coverslips and supplementing the growth medium with chemical modifiers (activin-a, BMP-4, insulin, valproic acid, and 5-azacytidine) in various combinations. The HA/G plasma gels were produced from human blood plasma-derived fibrinogen, gelatin, and human umbilical cord-derived hyaluronic acid. A networkbased approach was employed to select marker genes for cardiomyogenic differentiation, and the expression levels of three markers (GATA4, TBX5, and cTnI) were followed by RT-qPCR to investigate the cardiomyogenic differentiation potential of ASCs. Results indicated that each combination of chemical modifiers led to different expression levels in the .
