The present study addressed the successful in vitro seed germination, bulblet regeneration, increased bulblet size, hardening and acclimatization of Persian lily (Fritillaria persica). | Turkish Journal of Biology Turk J Biol (2016) 40: 878-888 © TÜBİTAK doi: Research Article Advancement in protocol for in vitro seed germination, regeneration, bulblet maturation, and acclimatization of Fritillaria persica 1 2 3 4 4, Derya ÇAKMAK , Cuma KARAOĞLU , Muhammad AASIM , Cengiz SANCAK , Sebahattin ÖZCAN * 1 Alanya District Directorate, Ministry of Food Agriculture and Livestock, Antalya, Turkey 2 Central Research Institute for Field Crops, Ministry of Food Agriculture and Livestock, Yenimahalle, Ankara, Turkey 3 Department of Biotechnology, Faculty of Science, Necmettin Erbakan University, Konya, Turkey 4 Department of Field Crops, Faculty of Agriculture, Ankara University, Ankara, Turkey Received: Accepted/Published Online: Final Version: Abstract: The present study addressed the successful in vitro seed germination, bulblet regeneration, increased bulblet size, hardening, and acclimatization of Persian lily (Fritillaria persica). Seed germination rate was recorded as and after 2 and 3 months of cold treatment at 4 °C, respectively. Bulblet explants taken from a germination experiment were cultured on Murashige and Skoog (MS) medium supplemented with – mg/L thidiazuron (TDZ). Maximum bulblet regeneration frequency and bulblets per explants were achieved on a medium containing mg/L TDZ. Leaf disc and leaf scale explants isolated from germinated seedlings were also cultured on MS medium containing 2–6 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) with a constant concentration of mg/L kinetin. Both explants yielded the highest bulblet regeneration on MS medium containing 2 mg/L 2,4-D and mg/L kinetin. A combination of g/L agar and g/L GELRITE resulted in a maximum bulblet diameter with a higher number of roots. The addition of 10 g/L NaCl to the culture medium resulted in the best root and leaf formation and secondary .
