This study examined the effect of yohimbine alone and in combination with glibenclamide on ischemia and reperfusioninduced arrhythmias in diabetes. Six minutes of ischemia were produced in 1-week diabetic rats by ligation of the left coronary artery and 6 min of reperfusion were produced by releasing the artery. | Turkish Journal of Biology Turk J Biol (2016) 40: 899-905 © TÜBİTAK doi: Research Article Short-term diabetes decreases ischemia reperfusion-induced arrhythmia: the effect of alpha-2 blocker yohimbine and glibenclamide 1, 2 1 1 Ömer BOZDOĞAN *, Salih Tunç KAYA , Selçuk YAŞAR , Erçin Çağdas BAŞ , 1 1 1 Oğulcan Talat ÖZARSLAN , Didem EKŞİOGLU , Firdevs ERİM 1 Department of Biology, Faculty of Science and Arts, Abant İzzet Baysal University, Gölköy, Bolu, Turkey 2 Department of Biology, Faculty of Science and Arts, Düzce University, Konuralp, Düzce, Turkey Received: Accepted/Published Online: Final Version: Abstract: This study examined the effect of yohimbine alone and in combination with glibenclamide on ischemia and reperfusioninduced arrhythmias in diabetes. Six minutes of ischemia were produced in 1-week diabetic rats by ligation of the left coronary artery, and 6 min of reperfusion were produced by releasing the artery. A dose of 5 mg/kg of yohimbine and glibenclamide was administered for 7 days prior to the coronary ligation. The blood pressure, heart rate, and arrhythmia were determined from the recorded ECG during the 6 min of ischemia and reperfusion and then compared using a one-way ANOVA statistical program and the chi-square test. The score of arrhythmia calculated from the type and duration of arrhythmia decreased in the diabetic rats ( ± in control, ± in diabetes group). The arrhythmia score returned to the control value in the rats that were given a combination of yohimbine and glibenclamide (3 ± ). Yohimbine is used in the treatment of erectile dysfunction; glibenclamide is used as an antidiabetic drug in diabetic patients and may be a risk factor in the increase of ischemia reperfusion-induced arrhythmias. Key words: Ischemia, reperfusion, arrhythmia, diabetes, glibenclamide, yohimbine 1. Introduction Ischemia that occurs .
