Suitability of 3 matrices, agarose, sodium alginate, and polyacrylamide, for immobilization of mutant cells of Pseudomonas aeruginosa MTCC 10,055 was investigated. Of these, agarose was proven to be the best as exhibiting maximum enzyme production ( U/mL), followed by polyacrylamide gel ( U/mL). | Turkish Journal of Biology Research Article Turk J Biol (2013) 37: 392-404 © TÜBİTAK doi: Optimization of immobilization conditions by conventional and statistical strategies for alkaline lipase production by Pseudomonas aeruginosa mutant cells: scale-up at bench-scale bioreactor level Deepali BISHT, Santosh Kumar YADAV, Nandan Singh DARMWAL* Department of Microbiology (Center of Excellence), Dr. Ram Manohar Lohia Avadh University, Faizabad, Uttar Pradesh, India Received: Accepted: Published Online: Printed: Abstract: Suitability of 3 matrices, agarose, sodium alginate, and polyacrylamide, for immobilization of mutant cells of Pseudomonas aeruginosa MTCC 10,055 was investigated. Of these, agarose was proven to be the best as exhibiting maximum enzyme production ( U/mL), followed by polyacrylamide gel ( U/mL). Alginate beads were the poorest. The one-variable-at-a-time approach suggested agarose at , immobilized bead at g blocks/50 mL, and initial cell loading of g in the matrix as optimum conditions for maximum lipase production ( U/mL) after 24 h of incubation. However, response surface methodology studies determined the optimum values of these variables as , g blocks/50 mL, and g of cells in the matrix for maximum lipase production ( U/mL) within h of incubation. The agarose blocks were reusable for 7 cycles without any significant loss in lipase yield. Bench-scale bioreactor level optimization resulted in further enhancement in lipase yield ( U/mL) at vvm aeration and 100 rpm agitation within only 20 h of incubation. Presumably, this is the first attempt for lipase production by immobilized cells of P. aeruginosa at the bioreactor level. The agarose-immobilized mutant cells showed potential candidature for alkaline lipase production at the industrial level. Key words: Alkaline lipase, .
