Cell suspension cultures of Taxus baccata were treated with 2 elicitor compounds, methyl jasmonate (MeJA) and squalestatin (S), individually and in combination for 7 days to determine if they mediated the enhancement of biosynthesis of endogenous jasmonate through induction of lipoxygenase (LOX) activity. | Turkish Journal of Biology Turk J Biol (2014) 38: 76-82 © TÜBİTAK doi: Research Article Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew 1 1,2, 1 1,2 3 Zohreh JALALPOUR , Leila SHABANI *, Ladan AFGHANI , Majid SHARIFI-TEHRANI , Sayed-Asadollah AMINI 1 Department of Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran 2 Research Institute of Biotechnology, Shahrekord University, Shahrekord, Iran 3 Department of Biochemistry, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran Received: Accepted: Published Online: Printed: Abstract: Cell suspension cultures of Taxus baccata were treated with 2 elicitor compounds, methyl jasmonate (MeJA) and squalestatin (S), individually and in combination for 7 days to determine if they mediated the enhancement of biosynthesis of endogenous jasmonate through induction of lipoxygenase (LOX) activity. Total phenolic compounds, total flavonoids, total antioxidants, phenylalanine ammonialyase (PAL), polyphenol oxidase (PPO), and LOX activities in 5-month-old yew cell cultures were studied. Our results showed that MeJA and S could stimulate production of phenol derivatives in cell suspension cultures of T. baccata. In parallel to the induction of phenolic production in elicited cells, results showed that activities of PAL and PPO enzymes and total antioxidants significantly increased in Taxus cells in response to MeJA and S. Maximal activities of lipoxygenase were obtained 48 h after treatment with MeJA (100 µM), S ( µM), and the combination of the 2 elicitors. Results showed that MeJA and S are effective elicitors for increasing phenolic production in Taxus cell suspension cultures, likely through increasing LOX activity followed by an increase in endogenous jasmonate. Key .
