An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. | Turkish Journal of Biology Turk J Biol (2014) 38: 371-379 © TÜBİTAK doi: Research Article Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.) 1 2 3 4 2 Handan BALTA , Özge KARAKAŞ METİN , Funda ŞENTÜRK AKFIRAT , Fahriye ERTUĞRUL , Semra HASANÇEBİ , 5 6 6 7 8, Yıldız AYDIN , Kadir AKAN , Zafer MERT , Müge TÜRET , Ahu ALTINKUT UNCUOĞLU * 1 Department of Biology, Faculty of Science, İstanbul University, İstanbul, Turkey 2 Genetic Engineering and Biotechnology Institute, TÜBİTAK Marmara Research Center, Gebze, Kocaeli, Turkey 3 Department of Molecular Biology and Genetics, Faculty of Science, Gebze Institute of Technology, Kocaeli, Turkey 4 Agriculture, Forestry and Veterinary Research Group, TÜBİTAK, Ankara, Turkey 5 Department of Biology, Faculty of Science and Arts, Marmara University, İstanbul, Turkey 6 Field Crop Research Institute, Lodumlu, Ankara, Turkey 7 Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Bogazici University, İstanbul, Turkey 8 Department of Bioengineering, Faculty of Engineering, Marmara University, İstanbul, Turkey Received: Accepted: Published Online: Printed: Abstract: An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. AFLP analysis was used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.) in this study. DNA isolated from the selected yellow rust tolerant and susceptible F2 individuals derived from a cross between Izgi2001 (resistant) and ES14 (susceptible) at seedling and adult stage was used for bulked segregant analysis combined with AFLP. From the screening of 34 PstI/MseI AFLP primer combinations, the AFLP marker P-GAC/M-ACG .
