Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta

Molecular techniques are used today in many areas and have increased the confidence level of studies. The aim of the study was to compare the classical morphological characters of coccoid green algae under field and culture conditions by using modern molecular analysis. | E. N. SOYLU, A. GÖNÜLOL Turk J Biol 36 (2012) 247-254 © TÜBİTAK doi: Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta Elif Neyran SOYLU1, Arif GÖNÜLOL2 1 Giresun University, Faculty of Arts and Science, Department of Biology, Giresun - TURKEY 2 Ondokuz Mayıs University, Faculty of Arts and Science, Department of Biology, Samsun - TURKEY Received: Abstract: Molecular techniques are used today in many areas and have increased the confidence level of studies. The aim of the study was to compare the classical morphological characters of coccoid green algae under field and culture conditions by using modern molecular analysis. To achieve this aim, we first isolated the coccoid green algae from Liman and Cernek lakes. The morphological variations of green algae were observed under culture conditions and the phylogenetic relationships of these strains were defined according to the 18S rRNA gene sequences. According to sequence analysis, 2 of the isolated species had high similarity to Scenedesmus subspicatus (98%) and Desmodesmus sp. (100%). Key words: Coccoid green algae, Scenedesmus subspicatus, molecular analysis, Desmodesmus sp., sequence analysis Kızılırmak Deltası’ndaki göllerden izole edilen küresel yeşil alglerin morfolojik ve 18S rRNA analizi Özet: Günümüzde moleküler teknikler pek çok alanda kullanılmakta ve yapılan çalışmaların güvenilirliğini artırmaktadır. Araştırmanın genel amacı, küresel yeşil alglerin klasik morfolojik karakterlerini arazi ve kültür şartları altında modern moleküler analizleri kullanarak karşılaştırmaktır. Bu amacı gerçekleştirebilmek için öncelikle Liman ve Cernek göllerinden (Samsun, Türkiye) alınan küresel yeşil alglerin farklı klon kültürlerinin izolasyonu gerçekleştirilmiştir. Kültür şartları altında yeşil alglerin morfolojik çeşitliliği gözlemlenerek, bu suşların 18S rRNA gen dizilerine göre filogenetik ilişkileri belirlenmiştir. DNA dizi .

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