Aerial parts of Daphne pontica were collected from Ilgaz-Çankırı, and D. oleoides subsp. oleoides was collected from 2 different localities (Ayrancı-Karaman and Ilgaz-Çankırı) in Turkey. The samples were subjected to hydrodistillation and microdistillation. | Turkish Journal of Biology Turk J Biol (2013) 37: 114-121 © TÜBİTAK doi: Research Article Comparison of the volatiles of Daphne pontica L. and D. oleoides Schreber subsp. oleoides isolated by hydro- and microdistillation methods 1, 2 3 İlhan GÜRBÜZ *, Betül DEMİRCİ , Gerhard FRANZ , 4 5 2 Kemal Hüsnü Can BAŞER , Erdem YEŞİLADA , Fatih DEMİRCİ 1 Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06300 Ankara, Turkey 2 Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, 26470 Eskişehir, Turkey 3 Department of Pharmaceutical Biology, Faculty of Pharmacy, Regensburg University, 93020 Regensburg, Germany 4 Department of Botany and Microbiology, College of Science, King Saud University, 11451 Riyadh, Saudi Arabia 5 Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, 34755 Kayışdağı, İstanbul, Turkey Received: Accepted: Published Online: Printed: Abstract: Aerial parts of Daphne pontica were collected from Ilgaz-Çankırı, and D. oleoides subsp. oleoides was collected from 2 different localities (Ayrancı-Karaman and Ilgaz-Çankırı) in Turkey. The samples were subjected to hydrodistillation and microdistillation. The resulting volatile samples were analyzed both by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS), respectively. The main components for D. pontica were identified as hexahydrofarnesyl acetone (), carvacrol (), dihydroedulane II (), (E)-geranyl acetone (), and thymol (), while nonacosane ( and ), hexadecanoic acid ( and ), phytol (), and carvacrol () were identified as the main components of D. oleoides subsp. oleoides obtained by hydrodistillation. Carvacrol (), thymol (), dihydroactinidiolide (), bicyclosesquiphellandrene (), and (Z)-3-hexenal () were the major components in D. pontica, while carvacrol .
