Ultraviolet B (UV-B) irradiation has an adverse effect on plant cells because it causes the generation of reactive oxygen species (ROS). This is scavenged by some endogenous defense systems. In this study, the activities of H2O2- detoxifying antioxidant enzymes in 4 barley cultivars pretreated with high temperature or NaCl before UV-B irradiation were investigated. | Turk J Biol 35 (2011) 59-68 © TÜBİTAK doi: Is the induction of H2O2-detoxifying antioxidant enzyme activities sufficient to protect barley cultivars from oxidative stress by UV-B irradiation alone or pretreatment with high temperature and NaCl? Hüsnü ÇAKIRLAR, Nuran ÇİÇEK, Yasemin EKMEKÇİ Hacettepe University, Faculty of Science, Department of Biology, Beytepe, 06800 Ankara - TURKEY Received: Abstract: Ultraviolet B (UV-B) irradiation has an adverse effect on plant cells because it causes the generation of reactive oxygen species (ROS). This is scavenged by some endogenous defense systems. In this study, the activities of H2O2detoxifying antioxidant enzymes in 4 barley cultivars pretreated with high temperature or NaCl before UV-B irradiation were investigated. The stress response in the seedlings was determined by measuring the photosynthetic performance index (PI), which significantly decreased in all barley cultivars under almost all treatments compared to their controls. Activities of 3 H2O2-detoxifying antioxidant enzymes (ascorbate peroxidase, glutathione reductase, and peroxidase) significantly increased as a result of all treatments. The results may suggest that UV-B irradiation induces major H2O2detoxifying enzyme activities, and pretreatments, especially with 200 mM of NaCl, enhance the response of enzyme activities in these 4 barley cultivars. Changes in the PI responses could indicate that the induction of antioxidant enzymes might not be enough to protect from oxidative stress induced by UV-B radiation in the barley cultivars investigated in the study. Key words: Ascorbate peroxidase, barley, glutathione reductase, high temperature, NaCl, peroxidase, photosynthetic performance index, UV-B irradiation Arpa çeşitlerinin yalnız veya yüksek sıcaklık ve NaCl ön uygulamalı UV-B ışımasıyla oluşan oksidatif stresten korunmak için H2O2 detoksifiye eden antioksidan enzim aktivitelerinin indüksiyonu yeterli midir? Özet: Ultra .
