The present study was conducted to determine the phenotypic and genotypic characterization of thermophilic bacteria isolated from Pasinler hot spring, Erzurum, Turkey. Fatty acid profiles, BOX PCR fingerprints, and 16S rDNA sequence data were used for the phenotypic and genotypic characterization of thermophilic bacteria. | A. ADIGÜZEL, K. İNAN, F. ŞAHİN, T. ARASOĞLU, M. GÜLLÜCE, A. O. BELDÜZ, Ö. BARIŞ Turk J Biol 35 (2011) 267-274 © TÜBİTAK doi: Molecular diversity of thermophilic bacteria isolated from Pasinler hot spring (Erzurum, Turkey) Ahmet ADIGÜZEL1, Kadriye İNAN2, Fikrettin ŞAHİN3, Tulin ARASOĞLU4, Medine GÜLLÜCE5, Ali Osman BELDÜZ2, Özlem BARIŞ5 1 Department of Molecular Biology and Genetics, Faculty of Science, Atatürk University, 25240, Erzurum - TURKEY 2 Department of Biology, Faculty of Arts and Science, Karadeniz Technical University, 61080 Trabzon - TURKEY 3 Department of Genetic and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, 4755 Kayışdağı, İstanbul - TURKEY 4 İstanbul Regional Hygiene Institute, Ministry of Health Turkey, 34020 Zeytinburnu, İstanbul - TURKEY 5 Department of Biology, Faculty of Science, Atatürk University, 25240 Erzurum - TURKEY Received: Abstract: The present study was conducted to determine the phenotypic and genotypic characterization of thermophilic bacteria isolated from Pasinler hot spring, Erzurum, Turkey. Fatty acid profiles, BOX PCR fingerprints, and 16S rDNA sequence data were used for the phenotypic and genotypic characterization of thermophilic bacteria. Totally 9 different bacterial strains were selected based on morphological, physiological, and biochemical tests. These strains were characterized by molecular tests including fatty acid and BOX profiles, and 16S rDNA sequence. The data of fatty acid analysis showed the presence of 14 different fatty acids in the 9 bacterial strains examined. Additionally, 5 of these fatty acids, 15:0 iso, 15:0 anteiso, 16:0 iso, 17:0 iso, and 17:0 anteiso fatty acids, were found in all isolates. Based on fatty acid profiles, it was determined that the bacterial strains were classified into 2 phenotypic groups, and these data were confirmed by BOX PCR genomic fingerprint profiles and 16S rDNA sequence analysis. The first group, .
