The purpose of the current study was to investigate the possible antimicrobial and anticancer properties of the aquatic moss Fontinalis antipyretica Hedw. (Fontinalaceae). We first obtained 8 different extracts (methanol, chloroform, acetone, ethyl acetate A, B, C, and D) by two different extraction processes. | F. SAVAROĞLU, C. FİLİK İŞÇEN, A. P. ÖZTOPCU VATAN, S. KABADERE, S. İLHAN, R. UYAR Turk J Biol 35 (2011) 361-369 © TÜBİTAK doi: Determination of antimicrobial and antiproliferative activities of the aquatic moss Fontinalis antipyretica Hedw. Filiz SAVAROĞLU1, Cansu FİLİK İŞÇEN2, Ayşe Pınar ÖZTOPCU VATAN1, Selda KABADERE3, Semra İLHAN1, Ruhi UYAR3 1 Department of Biology, Faculty of Art and Sciences, Eskişehir Osmangazi University, 26480 Eskişehir - TURKEY 2 Department of Elementary Education, Faculty of Education, Eskişehir Osmangazi University, 26480 Eskişehir - TURKEY 3 Department of Physiology, Faculty of Medicine, Eskişehir Osmangazi University, 26480 Eskişehir-TURKEY Received: Abstract: The purpose of the current study was to investigate the possible antimicrobial and anticancer properties of the aquatic moss Fontinalis antipyretica Hedw. (Fontinalaceae). We first obtained 8 different extracts (methanol; chloroform; acetone; ethyl acetate A, B, C, and D) by two different extraction processes. The antimicrobial activity of these extracts was then assessed by using the well diffusion method against 8 bacterial and 7 fungal strains. The results of antimicrobial studies showed that extracts of chloroform, acetone, and ethyl acetate A and C were active against almost all the species tested. The most effective extract was C, which was then screened through a preparative TLC (bioautography) for its active components. Spot 4 of extract C exhibited the highest antimicrobial activity against 5 bacterial strains (B. cereus, B. subtilis, E. faecalis, E. aerogenes, and Y. enterocolitica) and 3 fungal strains (A. flavus, F. solani, and F. graminearum), with MIC values of μg/mL and μg/mL, respectively. In addition, in vitro toxicity of the active component of extract C at the concentrations of , , 16, 80, and 160 μg/mL was tested. Depending on dose and time, only 80 and 160 μg/mL decreased rat glioma (C6) cell
