One-year-old olive (Olea europaea L. cv. Gemlik) seedlings were exposed to increasing levels of salinity in pot culture. The experiment was laid out in a randomized block design with 6 replicates. | M. A. DEMİRAL, D. AKTAŞ UYGUN, M. UYGUN, E. KASIRĞA, A. A. KARAGÖZLER Turk J Biol 35 (2011) 433-442 © TÜBİTAK doi: Biochemical response of Olea europaea cv. Gemlik to short-term salt stress Mehmet Ali DEMİRAL1, Deniz AKTAŞ UYGUN2, Murat UYGUN2, Erkan KASIRĞA1, Arife Alev KARAGÖZLER2 1 Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Adnan Menderes University, 09100 Aydın - TURKEY 2 Department of Chemistry, Faculty of Science and Arts, Adnan Menderes University, 09010 Aydın - TURKEY Received: Abstract: One-year-old olive (Olea europaea L. cv. Gemlik) seedlings were exposed to increasing levels of salinity in pot culture. The experiment was laid out in a randomized block design with 6 replicates. Dry matter (DM) percentage, sodium (Na) and chloride (Cl) concentrations, 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging activity, reducing power (RP), total phenolic content (TPC), and proline (PRO) content were determined in the leaves of the plants. The results showed that the olive plant used physiological and biochemical mechanisms consecutively to alleviate the effects of salt stress. The type of mechanism used was related to the severity of the salt stress. Salinity-induced variation in DPPH scavenging activity correlated well with the PRO and TPC variations of the leaves. The results showed that DPPH radical scavenging activity may be evaluated as a reliable parameter to assess the ability of antioxidants to hinder salt stress in olive plants. Key words: Chloride, dry matter, DPPH scavenging activity, reducing power, sodium, total phenolics Olea europaea cv. Gemlik bitkisinin kısa süreli tuz stresine biyokimyasal tepkisi Özet: Saksı kültüründe yetiştirilen bir yaşındaki zeytin (Olea europaea L. cv. Gemlik) fidanları artan dozlarda tuza maruz bırakılmıştır. Çalışma, tesadüf parselleri deneme deseninde ve 6 tekerrürlü olarak yürütülmüştür. Bitki yapraklarının kuru madde (KM) düzeyleri, sodyum (Na) ve klor .