Some hot springs located in the west of Turkey were investigated regarding the presence of xylanolytic thermophilic microorganisms. Based on phenotyping characteristics and 16S rRNA gene sequence analysis, 9 of these xylanolytic isolates belonged to the genus Anoxybacillus, and grew optimally at about 50-60 °C on nutrient agar | K. İNAN, S. ÇANAKÇI, A. O. BELDÜZ Turk J Biol 35 (2011) 529-542 © TÜBİTAK doi: Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey Kadriye İNAN, Sabriye ÇANAKÇI, Ali Osman BELDÜZ Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61080 Trabzon - TURKEY Received: Abstract: Some hot springs located in the west of Turkey were investigated regarding the presence of xylanolytic thermophilic microorganisms. Based on phenotyping characteristics and 16S rRNA gene sequence analysis, 9 of these xylanolytic isolates belonged to the genus Anoxybacillus, and grew optimally at about 50-60 °C on nutrient agar. The 16S rRNA gene sequence analysis showed that these isolates resembled the Anoxybacillus species ≥97 and these isolates are members of the genus Anoxybacillus. Based on the DNA-DNA hybridization study, SDS-PAGE profile, and biochemical and physiological features, I3, CT1Sari, and isolates are new strains of Anoxybacillus gonensis; and isolates are new strains of A. voinovskiensis; and , AC26, ACT14, and ACT2Sari isolates are new strains of A. kestanbolensis. The presence of xylanase activities, their optimum temperature, pH stability, and optimum pH were also investigated. The isolates ACT2Sari and ACT14 had the highest temperature optima (75 °C), and I3, CT1Sari, , ACT2Sari, and ACT14 had the highest pH optima (pH ) of xylanase. The xylanases of I3, CT1Sari, , , ACT2Sari, AC26, and ACT14 were optimally active both at alkaline pH and elevated temperature, and xylanases of , ACT2Sari, AC26, and ACT14 also were stable at alkaline pH. Key words: Alkaline xylanase, thermophiles, Anoxybacillus, 16S rRNA, DNA-DNA hybridization Türkiye’nin bazı kaplıcalarından ksilan kullanan yeni Anoxybacillus suşlarının izolasyonu ve karakterizasyonu Özet: Türkiye’nin batısında bulunan bazı kaplıcalarda, ksilan kullanan termofilik .
