In industrial hydrolysis processes the lignocellulose-degrading enzymes may have a potential application as an alternative to chemical treatments because they produce high specificity, mild reaction conditions, no toxic chemical hydrolysis inhibitors, and no loss of substrate due to chemical modifications. | Turk J Biol 33 (2009) 291-300 © TÜBİTAK doi: Co-operative actions and degradation analysis of purified lignocellulose-degrading enzymes from Thermomonospora fusca BD25 on wheat straw Münir TUNCER1, Andrew S. BALL2 1 Mersin University, Faculty of Arts and Science, Department of Biology, Çiftlikköy, 33342 Mersin - TURKEY 2School of Biological Sciences, Flinders University of South Australia GPO box 2100, Adelaide, South Australia 5001 – AUSTRALIA Received: Abstract: In industrial hydrolysis processes the lignocellulose-degrading enzymes may have a potential application as an alternative to chemical treatments because they produce high specificity, mild reaction conditions, no toxic chemical hydrolysis inhibitors, and no loss of substrate due to chemical modifications. The amounts of reducing sugars released from the degradation of wheat straw by the action of endoxylanase, peroxidase, β-xylosidase, and α-Larabinofuranosidase, which were produced by T. fusca BD25, were equal to , , , and hydrolysis (as xylose equivalents) of the substrate used, respectively, after 10 h of incubation. In the same conditions, the hydrolysis rate of endoglucanase, which was a commercial preparation from Trichoderma viride was . However, in order, addition of peroxidase, endoglucanase, β-xylosidase, and α-L-arabinofuranosidase preparations to the endoxylanase preparation significantly enhanced the reducing sugar yields (, , , and , respectively) and the hydrolysis of wheat straw (, , , and , respectively). The concentrations of phenolic-compounds as ligninequivalents released from wheat straw by the actions of lignocellulose-degrading enzyme preparations either acting alone or in combinations ranged from to μg mL-1 (as p-coumaric acid equivalents). The combination of purified lignocellulose-degrading enzymes significantly increased the degradation of straw to . The .
