To investigate the maternal donors and phylogenetic relationships of polyploids in Pseudoroegneria and related genera, the chloroplast trnL-F sequences of 31 Triticeae accessions were analyzed. The substitution saturation analysis of trnL-F sequences in this study suggested that they are suitable for phylogenetic analysis. | Turk J Biol 34 (2010) 335-342 © TÜBİTAK doi: Maternal donors of polyploids in Pseudoroegneria (Poaceae: Triticeae) and related genera inferred from chloroplast trnL-F sequences Yu HAI-QING1,2, Zhang CHUN1,2, Ding CHUN-BANG3, Ma XIAO1,2, Zhou YONG-HONG1,2* 1Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, Sichuan, CHINA 2Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan 625014, Sichuan, CHINA 3College of Biology and Science, Sichuan Agricultural University, Yaan 625014, Sichuan, CHINA Received: Abstract: To investigate the maternal donors and phylogenetic relationships of polyploids in Pseudoroegneria and related genera, the chloroplast trnL-F sequences of 31 Triticeae accessions were analyzed. The substitution saturation analysis of trnL-F sequences in this study suggested that they are suitable for phylogenetic analysis. In the trnL-F sequences tree, 4 major clades were formed: (a) the St/E clade comprised all of the Pseudoroegneria species together with species in Roegneria, Elytrigia, Douglasdeweya and Lophopyrum; (b) A. cristatum, A. mongolicum, and A. pectinatum subsp. retrofractum formed the P/W clade; (c) the Ns clade included species in Psathyrostachys; and (d) the H clade consisted of Hordeum species. The results suggested that: (a) diploid Pseudoroegneria species were the maternal donors of Stcontaining polyploid species in Pseudoroegneria, Roegneria, Douglasdeweya, and Elytrigia, and the trnL-F sequences were highly similar among them; (b) the trnL-F sequences of Agropyron species and Australopyrum species were similar, and the P genome was closely related to the W genome; and (c) the trnL-F sequences of species with the H or Ns genomes diverged greatly from that of species with the St, E, P, or W genomes. Key words: Pseudoroegneria, Douglasdeweya, Roegneria, Elytrigia, chloroplast trnL-F Pseudoroegneria (Poaceae: .
