Microbial biofilm and corrosion in cooling systems are the most common problems that damage expensive equipment, cause loss of production, and increase maintenance costs. Sulfate reducing bacteria were considered the major bacterial group involved in microbiologically influenced corrosion (MIC). | Turk J Biol 34 (2010) 389-397 © TÜBİTAK doi: Monitoring and disinfection of biofilm-associated sulfate reducing bacteria on different substrata in a simulated recirculating cooling tower system Esra İLHAN SUNGUR1,*, İrfan TÜRETGEN1, Reza JAVAHERDASHTI2, Ayşın ÇOTUK1 1Faculty of Science, Department of Biology, İstanbul University, 34134 Vezneciler, İstanbul - TURKEY 2Dept. of Petroleum Eng., Curtin University Senior Corrosion Engineer (Extrin) and Visiting Senior Lecturer / Researcher, Perth, AUSTRALIA Received: Abstract: Microbial biofilm and corrosion in cooling systems are the most common problems that damage expensive equipment, cause loss of production, and increase maintenance costs. Sulfate reducing bacteria were considered the major bacterial group involved in microbiologically influenced corrosion (MIC). We investigated the survival and enumeration of biofilm-associated SRB on coupons of galvanized steel, stainless steel, and copper, which are materials used in the manufacturing of cooling systems. We also investigated the effect of monochloromine on SRB as in mixedspecies mature biofilms formed on coupons by simulating recirculating cooling water conditions, due to the better penetration feature in biofilms than the residual chlorine. It was concluded that SRB count increased with time in bulk water and the surfaces (P < ). Experimental results supported by statistical analyses show that monochloromine is poorly effective on SRB colonies formed on galvanized and stainless steel surfaces. Key words: Sulphate reducing bacteria, biofilm, cooling tower, galvanized steel, stainless steel, copper Soğutma kulesi model sisteminde farklı tipte yüzeylerdeki biyofilmle ilişkili sülfat indirgeyen bakterilerin takibi ve dezenfeksiyonu Özet: Soğutma kulelerinde mikrobiyal biyofilm ve korozyon ekipmana zarar veren, üretim kaybına yol açan ve bakım maliyetlerini artıran hususlardır. Sülfat indirgeyen bakteriler (SRB) mikrobiyolojik .