The present study investigated the effect of methyleugenol, a food flavoring and fragrance agent, on the livers of laboratory rats. Doses of 10 and 30 mg/kg/day of methyleugenol (in methylcellulose) were administered intragastrically (IG) to 2 dose groups for 10 days alongside a control group (n = 10 for each group). | Turk J Biol 34 (2010) 439-445 © TÜBİTAK doi: Ultrastructural changes in rat liver by methyleugenol and evaluation of some biochemical parameters Ayşegül ÇERKEZKAYABEKİR1,∗, Gülnur KIZILAY2, Figen ERTAN1 1 Department of Biology, Trakya University, Science Faculty, 22080 Edirne - TURKEY 2Department of Histology & Embryology, Trakya University, School of Medicine, 22080 Edirne - TURKEY Received: Abstract: The present study investigated the effect of methyleugenol, a food flavoring and fragrance agent, on the livers of laboratory rats. Doses of 10 and 30 mg/kg/day of methyleugenol (in methylcellulose) were administered intragastrically (IG) to 2 dose groups for 10 days alongside a control group (n = 10 for each group). Gains in body weight were not statistically significant for either dose. Lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) activities decreased (P ). Liver Enzymes 600 Activity (U/mL) Histopathological procedures Body Weight Gain 350 300 500 Control 10 mg/kg 400 30 mg/kg 300 200 100 0 LDH/10 ALP AST ALT Figure 2. The effect of methyleugenol on LDH, ALP, AST, and ALT. Enzyme activities were measured in serum by using an autoanalyzer (Abbott, Architect C-8000). Decreases in LDH and ALP activities were statistically significant (P < ) for both dose groups, but AST activity was statistically significant (P < ) in only the 30 mg/kg/day dose group (arithmetic mean ± SD; standard deviation is given as an error bar). methyleugenol groups. ALT activity did not change significantly for either experimental group in comparison to the control group. Body weight values were not significantly different in the 10 mg/kg/day and 30 mg/kg/day methyleugenol groups compared to the body weight values of the control group. Body weight values are summarized in Figure 1. The total amounts of glucose and glycogen are shown in Figure 3. Decreases in glucose were statistically insignificant for both .
