Thirty nodule isolates from bean (Phaseolus vulgaris L.) grown in Eskiflehir were studied for their physiological and biochemical characteristics. Most isolates produced abundant extracellular polysaccharides, tolerated high salt concentration (5% NaCl), grew at a temperature of 42 °C, and synthesized melanin. | Turk J Biol 30 (2006) 127-132 © TÜB‹TAK Characterization of Rhizobium Sp. Isolated from Bean Çi¤dem KÜÇÜK1, Merih KIVANÇ2, Engin KINACI3 1Osmangazi 2Department University, Institute of Natural and Applied Sciences, Eskiflehir - TURKEY of Biology, Faculty of Science, Anadolu University 26470 Eskiflehir - TURKEY 3Osmangazi University, Faculty of Agriculture, Eskiflehir - TURKEY Received: Abstract: Thirty nodule isolates from bean (Phaseolus vulgaris L.) grown in Eskiflehir were studied for their physiological and biochemical characteristics. Most isolates produced abundant extracellular polysaccharides, tolerated high salt concentration (5% NaCl), grew at a temperature of 42 °C, and synthesized melanin. They were able to grow at pHs ranging from to . The majority of the isolates showed an intrinsic resistance to the antibiotics chloramphenicol (20 and 50 µg ml-1), erythromycin (30 µg ml-1), kanamycin (10 µg ml-1), and streptomycin (40, 80, and 100 µg ml-1). Plasmid DNA profiles of the isolates were identified and it was determined that 2 isolates contained plasmid DNA from 1 to 3. Key Words: Rhizobium sp., bean (Phaseolus vulgaris L.), isolation, isolate Fasülyeden ‹zole Edilen Rhizobium sp.’nin Karakterizasyonu Özet: Eskiflehir’de yetifltirilen fasulyelerden elde edilen otuz nodül izolat›n›n fizyolojik ve biyokimyasal özellikleri çal›fl›lm›flt›r. ‹zolatlar›n ço¤u ekstrasellüler polisakkarit üretmifl, yüksek tuz konsantrasyonuna (% 5 NaCl) toleransl› bulunmufl, 42 °C’lik s›cakl›kta geliflmifl ve melanin sentezlemifltir. ‹zolatlar 3,5’den 9’a kadar de¤iflen pH’da geliflebilmifltir. ‹zolatlar›n önemli bir k›sm› kloramfenikol (20 ve 50 µg ml-1), eritromisin (30 µg ml-1), kanamisin (10 µg ml-1) ve streptomisin (40, 80 ve 100 µg ml-1) antibiyotiklerine dirençlilik göstermifltir. ‹zolatlar›n plasmid DNA profileri incelenmifl ve iki izolat›n 1 ile 3 aras›nda plasmid DNA içerdi¤i belirlenmifltir. Anahtar Sözcükler: Rhizobium sp., Fasulye (Phaseolus
