Regenerated shoots formed complete plantlets on medium containing mg l-1 IBA, and mature plants were established, acclimatized, and thrived in greenhouse conditions. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of bioactive constituents of this medicinal plant. | Turk J Biol 32 (2008) 99-103 © TÜB‹TAK Plant Regeneration from Callus Culture of Clematis gouriana Roxb. – A Rare Medicinal Plant Hanumanaika RAJA NAIKA, Venkatarangaiah KRISHNA Plant Tissue Culture Laboratory, Department of . Studies and Research in Biotechnology and Bioinformatics, School of Biological Sciences, Kuvempu University, Shankaraghatta - 577 451, Karnataka - INDIA Received: Abstract: An in vitro regeneration protocol through stem callus culture has been standardized for the medicinal climber Clematis gouriana. The explant induced callus on MS-medium supplemented with to mg l-1 BAP and to mg l-1 NAA. The optimized callus induction occurred at the concentration of mg l-1 BAP and mg l-1 NAA. After initiation of callus, it was immediately transferred to MS medium containing mg l-1 FAP and mg l-1 indole-3-butyric acid (IBA). Upon longer incubation for about 2-6 weeks on the same culture medium initiation of shoot buds from the callus mass was observed and regeneration of plantlets with higher frequency (mean of ± shoots per explants) was noted. The microshoots rooted well on MS basal medium without growth regulators, as well as on medium supplemented with mg l-1 IBA. Regenerated shoots formed complete plantlets on medium containing mg l-1 IBA, and mature plants were established, acclimatized, and thrived in greenhouse conditions. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of bioactive constituents of this medicinal plant. Key Words: Clematis gouriana, medicinal climber, stem explant, plant regeneration Nadir Bulunan Bir T›bbi Bitki Olan Clematis gouriana Roxb.’un Kallus Kültüründen Bitki Rejenerasyonu Özet: Sarmafl›k türü olan Clematis gouriana t›bbi bitkisinin gövde kallus kültürü in vitro rejenerasyon çal›flmalar› için standardize edilmifltir. Bitkiden al›narak kültür ortam›na yerlefltirilen eksplantlarda, 0,5 .
