Adventitious shoots were obtained from the diploid Diyarbakir watermelon cultivar "Sürme" by culturing cotyledons on shoot regeneration medium for 3 weeks. The effects of two cytokinins, benzyl amino purine (BA) and kinetine (Kin), on shoot organogenesis were examined. The number of shoots per explant were approximately more than 50% higher when mg/l BA was used compared to the most effective kinetin concentration (1 mg/l). | Turk J Biol 27 (2003) 101-105 © TÜB‹TAK Adventitious Shoot Organogenesis and Plant Regeneration from Cotyledons of Diploid Diyarbak›r Watermelon (Citrullus lanatus cv. “Sürme”) Vedat P‹R‹NÇ* Department of Horticulture, Faculty of Agriculture, Dicle University, 21280 Diyarbak›r - TURKEY Ahmet ONAY Department of Biology Faculty of Science and Literature, Dicle University, 21280 Diyarbak›r - TURKEY Hakan YILDIRIM Department of Horticulture, Faculty of Agriculture, Dicle University, 21280 Diyarbak›r - TURKEY Filiz ADIYAMAN, Çi¤dem IfiIKALAN, Davut BAfiARAN Department of Biology Faculty of Science and Literature, Dicle University, 21280 Diyarbak›r - TURKEY Received: Abstract: Adventitious shoots were obtained from the diploid Diyarbak›r watermelon cultivar "Sürme" by culturing cotyledons on shoot regeneration medium for 3 weeks. The effects of two cytokinins, benzyl amino purine (BA) and kinetine (Kin), on shoot organogenesis were examined. The number of shoots per explant were approximately more than 50% higher when mg/l BA was used compared to the most effective kinetin concentration (1 mg/l). Plants were obtained by transferring 1 to 2 cm shoots to MS medium supplemented with 1-4 mg/l naphthalene acetic acid. Most of the regenerated plants (more than 50%) were successfully transplanted to the field. This demonstrates that in vitro propagation can be used to produce high quality diploid Diyarbak›r watermelon for use in breeding lines. Key Words: Regeneration, Tissue culture, Watermelon Diploid Diyarbak›r Karpuzu (Citrullus lanatus cv. "Sürme") Kotiledonlar›ndan Adventif Sürgün Organogenezisi ve Bitki Rejenerasyonu Özet: Diploid Diyarbak›r karpuzunun (Citrullus lanatus cv. "Sürme’’) kotiledonlar›ndan 3 haftal›k kültür sonucunda adventif sürgünler oluflturuldu. Sürgün organojenezisi üzerine iki tip sitokininin; benzil aminopürin (BA) ve kinetin (Kin) farkl› konsantrasyonlar›n›n etkileri araflt›r›ld›. Eksplant bafl›na oluflan sürgün say›s› .