t: In experiments with Escherichia coli B/r WP2(trpE-), mice marrow cells and human lymphocytes the antimutagenic activity of an extract from Zizyphus mill was performed. The extract was tested separately and in mixture with Triticum aestivum. | Turk J Biol 27 (2003) 137-143 © TÜB‹TAK Modification by Compositional Antimutagens of the Key Stages of a Mutation Process Urkhan ALEKPEROV, Ali ALIEV, Afet RUSTAMOVA Institute of Genetics, 155 Prospect Azadliq, 370106, Baku, Republic of Azerbaijan Saida JAFAROVA Baku State University, Z. Khalilov Street 23, 370145, Baku, Republic of Azerbaijan Received: Abstract: In experiments with Escherichia coli B/r WP2(trpE-), mice marrow cells and human lymphocytes the antimutagenic activity of an extract from Zizyphus mill was performed. The extract was tested separately and in mixture with Triticum aestivum. In experiments with different treatment schemes it has been established that the extract from wheat sprouts added to an antimutagenic preparation from Zizyphus mill intensifies the genoprotective properties of the Zizyphus mill extract. Key Words: mutation, antimutagens, plant extracts, chromosome aberrations, gene mutations Introduction As a result of intensive research in the field of genome protection, associated with the possibility for most antimutagens (AMs) to be used not only against the processes of induced mutagensis but also against carcinogenesis, more than 100 genoprotective agents are already known and their numbers are growing every year. Among these compounds, plant AMs are the most promising ones (1-7). The mechanisms by which AMs decrease mutation rates are different due to the specific characters of these compounds to influence particular stages of the mutation formation process. It has been shown that these compounds may inactivate genotoxicants or inhibit the metabolic activation of promutagens as well as correct the mutation process through influencing the process of DNA replication and repair (710). The efficiency of AMs is largely determined by the genoprotective agent’s ability to participate in the correction, at least, of some key stages of multistage mutation processes. The solution to the problem of how to achieve increases
