The amylase producer thermophilic Bacillus sp. K-12 was isolated from soil samples from Zeytinli hot spring in Kahramanmarafl. Enzyme synthesis occurred at 20-55 ºC with an optimum of 42 ºC. There was a slight variation in amylase synthesis within the pH range . | Turk J Biol 29 (2005) 99-103 © TÜB‹TAK Effects of Carbon Sources and Various Chemicals on the Production of a Novel Amylase from a Thermophilic Bacillus sp. K-12 Özlem KIRAN1, U¤ur ÇÖMLEKÇ‹O⁄LU1, Burhan ARIKAN2 1 Kahramanmarafl Sütçü ‹mam University, Faculty of Arts and Sciences, Department of Biology, Kahramanmarafl - TURKEY 2 Çukurova University, Faculty of Arts and Sciences, Department of Biology, Adana - TURKEY Received: Abstract: The amylase producer thermophilic Bacillus sp. K-12 was isolated from soil samples from Zeytinli hot spring in Kahramanmarafl. Enzyme synthesis occurred at 20-55 ºC with an optimum of 42 ºC. There was a slight variation in amylase synthesis within the pH range . Effects of various carbon sources and chemicals on α-amylase production were examined and maximum α-amylase production was obtained in a medium containing 1% starch in 60 h. MnSO4, ZnSO4 and EDTA inhibited α-amylase production of Bacillus sp. K-12. Key Words: Thermostable, Amylase, Bacillus Termofilik Bacillus sp. K-12’nin, Yeni Bir Amilaz Üretimi Üzerine Karbon Kaynaklar› ve Çeflitli Kimyasallar›n Etkisi Özet: Amilaz üreticisi termofilik Bacillus sp. K-12 suflu Kahramanmarafl’ta bulunan Zeytinli Il›cas›’ndan al›nan toprak örneklerinden izole edilmifltir. Enzim sentezi 20-55 ºC s›cakl›klar› aras›nda gerçekleflirken optimum s›cakl›k 42 ºC olarak bulunmufltur. Amilaz sentezinde 4,5-10,5 pH aral›klar›nda de¤iflimler görülmüfltür. Amilaz üretimi üzerine çeflitli karbon kaynaklar› ve kimyasallar›n etkisi incelenmifltir. Maksimum α-amilaz üretimi %1 niflasta içeren besiyerinde 60. saatte elde edilmifltir. MnSO4, ZnSO4 ve EDTA, Bacillus sp. K-12’in amilaz üretimini inhibe etti¤i görülmüfltür. Anahtar Sözcükler: Termal Kararl›, Amilaz, Bacillus Introduction Starch, the primary storage polysaccharide in plants, is degraded by amylolytic enzymes from numerous microorganisms (1). Amylases from plants, animals and microorganisms have been studied since enzymes .
