In the present study, the carbohydrate-binding module (CBM) coding sequences of the cellulosomes of 13 thermophilic, cellulolytic, anaerobic, ethanol-producing bacterial isolates having some variations in their growth and cellulose degradation capacities were amplified by PCR and then sequenced. | Turk J Biol 30 (2006) 45-50 © TÜB‹TAK Primary Structure of the Carbohydrate-Binding Modules in Various Cellulolytic, Thermophilic, Anaerobic, Ethanol-Producing Isolates 1 2 Melek ÖZKAN , Gülay ÖZCENG‹Z 1 Department of Environmental Engineering, Gebze Institute of Technology, 41400 Kocaeli - TURKEY 2 Department of Biology, Middle East Technical University, 06531 Ankara - TURKEY Received: Abstract: In the present study, the carbohydrate-binding module (CBM) coding sequences of the cellulosomes of 13 thermophilic, cellulolytic, anaerobic, ethanol-producing bacterial isolates having some variations in their growth and cellulose degradation capacities were amplified by PCR and then sequenced. The sequence analysis of the amplicons revealed that CBMs of 7 of the isolates including the isolate 7-9-1 with the highest capacity of cellulose degradation in solid medium have 100% identity in both nucleotide and amino acid sequences to CipB of Clostridium thermocellum in the compared regions. On the other hand, CBM of the isolate 7-1-2, also having a high cellulolytic activity, was found to differ for as much as 66 amino acid residues out of 100 (66%). The isolate 7-9-4 with a relatively low cellulose-degrading capacity also displayed amino acid variation for this protein, but only for 4 out of 118 residues. Key Words: Cellulose degradation, carbohydrate-binding modules, thermophilic and cellulolytic bacteria, Clostridium thermocellum Çeflitli Selülolitik, Termofilik, Anaerobik, Etanol Üreticisi ‹zolatlarda Karbonhidrat Ba¤lanma Modüllerinin Birincil Yap›lar› Özet: Bu çal›flmada üreme ve selüloz kullan›m kapasiteleri bak›m›ndan baz› farkl›l›klar gösteren 13 ayr› termofilik, selülolitik, anaerobik, etanol üreticisi bakteri izolat›n›n selülozomlar›n›n CBM kodlayan bölgeleri PCR ile ço¤alt›lm›fl ve DNA dizileri saptanm›flt›r. Amplikonlar›n k›yaslanan bölgelerinin DNA dizi analizleri, kat› besiyerinde en yüksek selüloz kullan›m kapasitesine sahip 7-9-1 suflunu da
