In this work, Pediococcus acidilactici F isolated from fermented sausage was reported to produce an extracellular antimicrobial peptide, termed Pediocin F, that was protein in nature and effective against many bacteria associated with food spoilage and food related health hazards. | Tr. J. of Biology 22 (1998) 217-228 © TÜBİTAK Purification and Characterization of Pediocin F, A Bacteriocin Produced By Pediococcus acidilactici F Özlem OSMANAĞAOĞLU Gazi University, Faculty of Arts and Science, Department of Biology Teknikokullar, 0650 Ankara -TURKEY Ufuk GÜNDÜZ Middle East Technical University, Faculty of Arts and Science, Department of Biology, Ankara-TURKEY Yavuz BEYATLI Gazi University, Faculty of Arts and Science, Department of Biology, Teknikokullar, 06500 Ankara-TURKEY Cumhur ÇÖKMÜŞ Ankara University, Faculty of Science, Department of Biology, 06100 Ankara-TURKEY Received: Abstract: In this work, Pediococcus acidilactici F isolated from fermented sausage was reported to produce an extracellular antimicrobial peptide, termed Pediocin F, that was protein in nature and effective against many bacteria associated with food spoilage and food related health hazards. Pediocin F is reported here to be sensitive to proteolytic enzymes, resistant to heat and organic solvents, and active over a wide range of pH. We have also reported that the extracellular antimicrobial protein produced by P. acidilactici F was really a small peptide. Coomassie-blue stained SDS-PAGE technique allowed us to estimate the molecular weight of this small peptide to be about 4460 dalton. Key Words: Pediococcus acidilactici, Pediocin, characterization, SDS-PAGE Pediococcus acidilactici F Tarafından Üretilen Pediocin F’ in Saflaştırılıp Karakterize Edilmesi Özet: Bu çalışmada fermente sosisden izole edilen Pediococcus acidilactici F’ in protein yapısında olan, gıdalarla ilgili sağlığı tehtit eden ve gıdaların bozulmasına sebep olan bir çok bakteriye karşı Pediocin F olarak adlandırılan hücre dışı bir antimikrobiyal peptit ürettiği rapor edilmiştir. Pediocin F, proteolitik enzimlere karşı hassas, ısıya ve organik çözücülere karşı dayanıklı ve geniş bir pH skalasında aktiftirler. Coomassie-blue ile boyanmış SDS-PAGE tekniği bakterinin antimikrobiyal etkisi ile
