The effect of kinetin, gibberellic acid and indole acetic acid on EMS-induced somatic mutation and recombination in Drosophila melanogaster

The effect of plant growth hormones (kinetin, gibberellic acid (GA3) and indole acetic acid (IAA)) on EMS-induced mutant wing spots was studied with the somatic mutation and recombination test (SMART) in Drosophila melanogaster. | Turk J Biol 24 (2000) 279–284 © TÜBİTAK The Effect of Kinetin, Gibberellic Acid and Indole Acetic Acid on EMS-Induced Somatic Mutation and Recombination in Drosophila melanogaster Elif YEŞİLADA Department of Biology, Art and Science Faculty, İnönü University, 44069 Malatya-TURKEY Received: Abstract: The effect of plant growth hormones (kinetin, gibberellic acid (GA3) and indole acetic acid (IAA)) on EMS-induced mutant wing spots was studied with the somatic mutation and recombination test (SMART) in Drosophila melanogaster. GA3 reduced all kinds of EMS-induced spot. While a 10-3 M concentration of kinetin reduced only the number of EMS-induced twin spots, a 10-4 M concentration was seen to increase the number of all types of spot. The same concentrations of IAA gave variable results. A 10-4 M concentration of IAA caused a decrease only in the number of large single spots. Our results confirmed that plant growth hormones, especially GA3, can act as a bio-antimutagen. Key Words: Ethyl Methanesulphonate, Gibberellic Acid, Indole Acetic Acid, Kinetin, Antimutagenicity, Somatic Mutation and Recombination Test, Drosophila melanogaster. Drosophila meanogaster’de EMS ile İndüklenmiş Somatik Mutasyon ve Rekombinasyon Üzerine Kinetin, Gibberellik Asit ve İndol Asetik Asitin Etkisi Özet: Bu çalışmada, Drosophila melanogaster’in somatik mutasyon ve rekombinasyon testi kullanılarak EMS ile indüklenmiş mutant kanat benekleri üzerine bitki büyüme hormonlarının (kinetin, gibberellik asit (GA3) ve indol asetik asit (IAA)) etkisi araştırıldı. GA3, EMS ile indüklenen bütün kanat beneklerini azalttı. Kinetinin 10-3 M konsantrasyonu EMS ile indüklenen ikili beneklerin sayısını azaltırken, 10-4 M konsantrasyonu beneklerin bütün tiplerinin sayısında artışa neden oldu. Aynı konsantrasyonlardaki IAA ise değişken sonuçlar verdi. 10-4 M IAA yalnızca büyük tekli beneklerin sayısını indirgedi. Sonuçlarımız bitki büyüme hormonlarının (özellikle GA3’ün) bio-antimutagen .

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