Protein profiles and prevalence of Methicillin resistant Staphylococcus aureus (MRSA) in Gülhane military academy hospital in Turkey

The present study was undertaken to determine the relationship between MethicillinResistant Staphylococcus aureus (MRSA) strains isolated from various clinical specimens in Gülhane Military Medical Academy, in Ankara, Turkey. | Turk J Biol 24 (2000) 809–816 © TÜBİTAK Protein Profiles and Prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) in Gülhane Military Academy Hospital in Turkey Sefa C. SAÇILIK Ankara University, Faculty of Science, Department of Biology, 06100 Tandoğan, Ankara-TURKEY Özlem OSMANAĞAOĞLU Gazi University, Faculty of Arts and Science, Department of Biology, 06500 Teknikokullar, Ankara-TURKEY Özgül KISA, Ahmet BAŞUSTAOĞLU Gülhane Military Medical Academy, Department of Clinical Microbiolog, Etlik, Ankara-TURKEY Cumhur ÇÖKMÜŞ Ankara University, Faculty of Science, Department of Biology, 06100 Tandoğan, Ankara-TURKEY Received: Abstract: The present study was undertaken to determine the relationship between MethicillinResistant Staphylococcus aureus (MRSA) strains isolated from various clinical specimens in Gülhane Military Medical Academy, in Ankara, Turkey. In 1997, at this hospital, the percentage of S. aureus among all nosocomial isolates was 20% and the percentage of MRSA among these S. aureus isolates was 51%. Both sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Native-Page (N-PAGE) were used for typing MRSA isolates which have common antibiotic susceptibility test results. MRSA isolates were also evaluated according to the whole-cell proteins (WCPs) and filtrate proteins (FPs). Using these methods, we concluded that all of the MRSA strains are from the same clonal group. These results are consistent with the results of our previous study. Gülhane Askeri Tıp Akademisi’ndeki Metisilin Dirençli Staphylococcus aureus (MRSA) Suşlarının Yayılımı ve Protein Profilleri Özet: Bu çalışmamızda Gülhane askeri Tıp Akademisi’ndeki değişik klinik örneklerden izole edilen MRSA suşları arasındaki ilişki belirlenmiştir. 1997 yılında, bu hastanedeki bütün nosokomiyal izolatlar arasında S. aureus oranı %20, bu S. aureus’lar arasındaki MRSA (Metisilin dirençli Staphylococcus aureus) oranı ise %50 olarak belirlenmiştir. Ortak .

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