In this study, we evaluated the application of green fluorescent protein (GFP) from the jellyfish Aequorea victoria, for efficient selection of possible transgenic and non-mosaic mouse embryos after microinjection. | Turk J Biol 25 (2001) 123-131 © TÜB‹TAK Application of Green Fluorescent Protein as a Marker for Selection of Transgenic Mouse Embryos before Implantation Haydar BA⁄Ifi TÜB‹TAK, Marmara Research Center, Research Institute for Genetic Engineering and Biotechnology, . Box 41, 41470 Gebze, Kocaeli-TURKEY Levent KESK‹NTEPE Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta GA 30912-2600, USA. Received: Abstract: In this study, we evaluated the application of green fluorescent protein (GFP) from the jellyfish Aequorea victoria, for efficient selection of possible transgenic and non-mosaic mouse embryos after microinjection. We injected 353 one-cell mouse zygotes with a DNA fragment carrying Gfp gene under the control of ß-actin gene promoter. Eighty-seven per cent (307 embryos) of the injected embryos survived after microinjection. The surviving embryos were cultured for an additional day and then analysed for detection of GFP expression using a standard fluorescein isothiocyanate (FITC) filter set attached to a microscope. In this analysis, 34 % of the two-cell stage embryos (37 embryos out of 108) were found to be GFP positive. Of these GFP-positive embryos, 8 embryos out of 37 (21%) were found to be non-mosaic as GFP expression was detected in all the blastomeres. Our results show that the successful use of GFP as a vital marker for detection of mosaic embryos holds promise for potential application of this technology as a selection tool in the generation of transgenic animals. Key Words: Transgenic mice, embryo, microinjection, green fluorescent protein Transgenik Fare Embriyolar›n›n ‹mplantasyon Öncesi Seçiminde Yeflil Floresans Proteini'nin Mark›r Olarak Kullan›m› Özet: Bu çal›flmada, Aequrea victoria (deniz anasi) dan elde edilen yeflil floresan proteinin (GFP) ekspresyonunun mark›r olarak kullan›lma olana¤›, mikroenjeksiyon sonras› elde edilen transgenik ve mozaik olmayan fare embriyolar›nda araflt›r›ld›. Bu .
