Isolation and characterization of pediocin producing Pediococcus pentosaceus Pep1 from vacuum packed sausages

Pediococcus pentosaceus Pep1 was isolated from vacuum-packed sausages and it was shown to produce a potentially novel antimicrobial agent active against several species of Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, Staphylococcus, Enterococcus, Bacillus and Listeria, many of which are associated with food spoilage and health hazards of food origin. | Turk J Biol 25 (2001) 133-143 © TÜB‹TAK Isolation and Characterization of Pediocin Producing Pediococcus pentosaceus Pep1 from Vacuum-Packed Sausages Özlem OSMANA⁄AO⁄LU Gazi University, Faculty of Arts and Science, Department of Biology, 06500, Teknikokullar, Ankara - TURKEY Yavuz BEYATLI Gazi University, Faculty of Arts and Science, Department of Biology 06500, Teknikokullar, Ankara - TURKEY Ufuk GÜNDÜZ Middle East Technical University, Faculty of Arts and Science Department of Biology, 06530, Ankara-TURKEY Received: Abstract : Pediococcus pentosaceus Pep1 was isolated from vacuum-packed sausages, and it was shown to produce a potentially novel antimicrobial agent active against several species of Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, Staphylococcus, Enterococcus, Bacillus and Listeria, many of which are associated with food spoilage and health hazards of food origin. Gramnegative organisms such as Yersinia enterocolitica and Escherichia coli were not inhibited by this antimicrobial agent. Since the antimicrobial agent was found to be sensitive to proteolytic enzymes, resistant to heat and organic solvents and active over a wide range of pH values, between 3 and 8, apparently most stable in the lower part of that range, it was identified as a bacteriocin and was designated as pediocin P. Key Words: Vacuum-packed sausages, Pediococcus pentosaceus, pediocins, bacteriocins, characterization Vakum ile Paketlenmifl Sosis Örneklerinden Pediosin Üreten Pediococcus pentosaceus Pep1 Suflunun ‹zolasyonu ve Karekterizasyonu Özet : Vakum ile paketlenmifl sosis örneklerinden izole edilen Pediococcus pentosaceus Pep1 suflunun Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, Staphylococcus, Enterococcus, Bacillus ve Listeria gibi birçok türü g›da bozulmalar›na ve g›da kökenli hastal›klara sebebiyet veren bakterilere karfl› potansiyel bak›mdan yeni olarak tan›mlanabilecek antimikrobiyal bir madde üretti¤i tespit edilmifltir. Gram-negatif .