Seven cultivar varieties of Vicia sativa L. were used. Nucleotypic effects of telophase (2C) nuclear DNA amounts on seed weight, percent mitotic index, stomatal frequency/mm2 chlorophyll a and b content, root growth and shoot growth were determined. | Turk J Biol 25 (2001) 205-219 © TÜB‹TAK Nucleotypic Effects in Different Genotypes of Vicia sativa L. Serap ÇEL‹KLER, Rahmi B‹LALO⁄LU Uluda¤ University, Faculty of Science and Letter, Department of Biology, Görükle Campus, Bursa - TURKEY Received: Abstract: Seven cultivar varieties of Vicia sativa L. were used. Nucleotypic effects of telophase (2C) nuclear DNA amounts on seed weight, percent mitotic index, stomatal frequency/mm2, chlorophyll a and b content, root growth and shoot growth were determined. No correlation was observed between the DNA contents and the percent mitotic index, chlorophyll a and b content, or root growth of the cultivar varieties. However a significant correlation was observed between DNA content and the seed weight, stomatal frequency and shoot growth of the cultivar varieties. Key Words: Vicia sativa, Nuclear DNA Content, Nucleotypic Effect De¤iflik Vicia sativa L. Genotiplerinde Nükleoptik Etkiler Özet: Vicia sativa L'nin 7 çeflit ve hatt› kullan›lm›flt›r. Telofaz (2C) DNA miktarlar›n›n, tohum a¤›rl›¤›, % mitotik indeks, stoma frekans›/mm2, klorofil a ve b içeri¤i, kök büyümesi ve gövde büyümesi üzerine olan etkileri araflt›r›lm›flt›r. Kullan›lan çeflit ve hatlar›n DNA miktar› ile % mitotik indeks, klorofil a ve b içeri¤i ve kök büyümesi aras›nda anlaml› bir iliflki olmad›¤›, fakat DNA miktar› ile tohum a¤›rl›¤›, stoma frekans› ve gövde büyümesi aras›nda anlaml› bir iliflki oldu¤u belirlenmifltir. Anahtar Sözcükler: Vicia sativa, Çekirdek DNA Miktar›, Nükleotipik Etki Introduction Study of the nuclear DNA content of different plant species generally reveals that the DNA content of each genome is constant and species-specific (1, 2). Microspectrophotometric determinations have shown that if the nuclear DNA content per genome is different in families and species of the same family, then it is also different between different varieties of the same species (3-11). Such great variation is known to exist within angiosperm .
