Thirty-eight alkalitolerant-mesophilic Streptomyces, defined by Saddler in his numerical taxonomic study, were evaluated by gas chromotography and pyrolysis mass spectrometry. Group I Streptomyces sp. AK409 and group II Streptomyces sp. AK029 selected duplicated strains were clustered in their own groups. | Turk J Biol 25 (2001) 463-475 © TÜB‹TAK The Evaluation of Alkalitolerant-Mesophilic Streptomyces by Gas Chromatography and Pyrolysis Mass Spectrometry Nevzat fiAH‹N, Kamil IfiIK, Ergin KAR‹PTAfi . Fen Edebiyat Fakültesi, Biyoloji Bölümü, 55139 Kurupelit-Samsun - TURKEY Michael GOODFELLOW Newcastle University, Medical School, Microbiology Dept., Newcastle upon Tyne - . Received: Abstract: Thirty-eight alkalitolerant-mesophilic Streptomyces, defined by Saddler in his numerical taxonomic study, were evaluated by gas chromotography and pyrolysis mass spectrometry. Group I Streptomyces sp. AK409 and group II Streptomyces sp. AK029 selected duplicated strains were clustered in their own groups. It was observed that the results of gas chromatography and pyrolysis mass spectrometry was in good congruance with the numerical taxonomic results. Therefore, it can be concluded that the different chemotaxonomic methods provide rapid and reproducible information for classifying biotechnologically significant Streptomyces strains. Key Words: Streptomyces, Gas chromotography, PyMS, Chemotaxonomy. Pirolizis Kütle Spektrometri ve Gaz Kromatografisi ile AlkalitolerantMezofilik Streptomyces’lerin De¤erlendirilmesi Özet: Saddler taraf›ndan nümerik taksonomisi yap›lan alkalitolerant-mezofilik otuz sekiz Streptomyces suflu gaz kromatografi ve pirolizis kütle spektrometri metotlar› ile de¤erlendirildi. Grup I’ den Streptomyces sp. AK409 ve grup II’den Streptomyces sp. AK029 nolu seçilen duplikat sufllar ilgili gruplar› ile kümelendiler. Gaz kromatografisi ve pirolizis kütle spektrometri sonuçlar›n›n nümerik taksonomik sonuçlarla tam bir uyum içerisinde oldu¤u gözlendi. Farkl› kemotaksonomik metotlar›n biyoteknolojik önemi olan Streptomyces sufllar›n›n s›n›fland›r›lmas›nda h›zl› ve üretken bilgiler sa¤lad›¤› sonucuna var›lmaktad›r. Anahtar Sözcükler: Streptomyces, Gaz kromatografisi, PyMS, Kemotaksonomi. Introduction The genus Streptomyces is now well circumscribed
