Hypocotyl and stem explants excised from in vitro and greenhouse-grown seedlings of Madaras, 1886 Sel. and Omega flax cultivars were compared in terms of percentage of explants producing shoots, shoot number per explant and total shoot number per petri dish. Hypocotyl explants resulted in better shoot regeneration. | Turk J Biol 26 (2002) 37-40 © TÜB‹TAK The Effect of Different Explant Sources on Adventitious Shoot Regeneration in Flax (Linum usitatissimum L.) Mustafa YILDIZ, Sebahattin ÖZCAN and Celâl ER Ankara University, Faculty of Agriculture, Department of Field Crops, 06110 Ankara - TURKEY Received: Abstract: Hypocotyl and stem explants excised from in vitro and greenhouse-grown seedlings of Madaras, 1886 Sel. and Omega flax cultivars were compared in terms of percentage of explants producing shoots, shoot number per explant and total shoot number per petri dish. Hypocotyl explants resulted in better shoot regeneration. In vitro-grown seedlings were found to be more suitable than greenhouse-grown seedlings as an explant source. Key Words: Explant sources, adventitious shoot regeneration, flax Keten (Linum usitatissimum L.)’de Adventif Sürgün Rejenerasyonu Üzerine Farkl› Eksplant Kaynaklar›n›n Etkisi Özet: Madaras, 1886 Sel. ve Omega çeflitlerine ait olup, in vitro ve serada yetifltirilen fidelerden al›nan hipokotil ve sap eksplantlar›, sürgün veren explant yüzdesi, eksplant bafl›na sürgün say›s› ve petrideki toplam sürgün say›s› bak›m›ndan karfl›laflt›r›lm›flt›r. Eksplantlar aras›nda hipokotil en iyi rejenerasyonu vermifltir. Eksplant kayna¤› olarak in vitro yetifltirilen fideler, serada yetilfltirilen fidelere göre daha uygun bulunmufltur. Anahtar Sözcükler: Eksplant kaynaklar›, adventif sürgün rejenerasyonu, keten Introduction The aim of genetic transformation experiments is to regenerate whole fertile transformed plants. The adventitious shoot regeneration capacity of cells or tissues to be used in transformation studies affects the success of gene transformation significantly (1-3). Some dicotyledonous plants from the families Solanaceae, Begoniaceae, Crassulaceae, Gesneriaceae and Cruciferae regenerate more easily in vitro. Flax has a good regeneration system (4-6). However, the production of transformed flax plants from transformed callus is very .
