Morphology and phylogenetic analyses using the ITS and LSU regions of nrDNA were carried out on bolete collections belonging to Boletus s. str., originating from the Himalayan region of Pakistan. The phylogenetic analysis outlined the existence of previously unknown species of this genus. It is described here as Boletus himalayensis. | Turkish Journal of Botany Turk J Bot (2018) 42: 790-800 © TÜBİTAK doi: Research Article Boletus himalayensis (Basidiomycota; Boletales), a new porcini species from Pakistan 1, 2 3 4 5 Samina SARWAR * , Sana JABEEN , Ishtiaq AHMAD , Bryn M. DENTINGER , Abdul Nasir KHALID 1 Department of Botany, Lahore College for Women University, Lahore, Pakistan 2 Department of Botany, Division of Science and Technology, University of Education, Township, Lahore, Pakistan 3Centre of Plant Biodiversity, University of Peshawar, Peshawar, Pakistan 4 Natural History Museum of Utah, University of Utah, Salt Lake City, UT, USA 5 Department of Botany, University of the Punjab, Lahore, Pakistan Received: Accepted/Published Online: Final Version: Abstract: Morphology and phylogenetic analyses using the ITS and LSU regions of nrDNA were carried out on bolete collections belonging to Boletus s. str., originating from the Himalayan region of Pakistan. The phylogenetic analysis outlined the existence of previously unknown species of this genus. It is described here as Boletus himalayensis. Illustrations of the macroscopic characters and the microscopic features of the new species are provided. The distinction of B. himalayensis from allied species is discussed. Key words: Boletus s. str., molecular analysis, morphology, phylogeny, taxonomy 1. Introduction The Himalayan region is considered a biodiversity-rich hotspot and an ecosystem of global importance. Despite its importance, this region is one of the least studied in terms of the diversity of macrofungi (Mirjam, 2010). The boletes are no exception. Bolete diversity from the entire Himalayan region is only represented by some sporadic publications (Ahmad et al., 1997; Das et al., 2012; Das, 2013a, 2013b; Das and Chakraborty, 2014; Sarwar et al., 2011; 2012; 2014a, 2014b, 2014c, 2015, 2016; HernándezRestrepo et al., 2016). The present .
