Hemianthus callitrichoides ‘Cuba’ is an ornamental aquatic plant used in water gardens and phytoremediation. A protocol for its rapid and reproducible shoot organogenesis from apical shoot explants was developed. | Turkish Journal of Biology Turk J Biol (2015) 39: 493-500 © TÜBİTAK doi: Research Article In vitro high frequency regeneration through apical shoot proliferation of Hemianthus callitrichoides ‘Cuba’ ‐ a multipurpose ornamental aquatic plant 1, 2 3 1 Surendra BARPETE *, Sancar Fatih ÖZCAN , Muhammad AASIM , Sebahattin ÖZCAN Department of Field Crops, Faculty of Agriculture, Ankara University, Dışkapı, Ankara, Turkey 2 Central Research Institute for Field Crops, Ministry of Food Agriculture and Livestock, Yenimahalle, Ankara, Turkey 3 Department of Biology, Kamil Özdağ Faculty of Science, Karamanoğlu Mehmetbey University, Karaman, Turkey 1 Received: Accepted/Published Online: Printed: Abstract: Hemianthus callitrichoides ‘Cuba’ is an ornamental aquatic plant used in water gardens and phytoremediation. A protocol for its rapid and reproducible shoot organogenesis from apical shoot explants was developed. Optimum culture conditions for shoot proliferation were tested in MS media containing different concentrations of 6-(γ,γ-dimethylallylamino)purine (2iP), 6-benzylaminopurine (BAP), 1-phenyl-3-1,2,3-thiadiazol-5-yl urea (TDZ), and gibberellic acid (GA3). Plant growth regulators significantly (P ≤ ) affected the diameter, area, and fresh weight of shoot clumps. On the basis of plant growth, the maximum shoot diameter ( cm), area ( cm), and clump weight ( g) of apical shoots were achieved on MS medium containing mg L–1 BAP. Higher concentrations of sucrose (9%) showed visible phenotypic growth inhibition and abnormalities were observed in vitro. The regenerated shoots rooted efficiently on MS medium without growth regulators. The growth pattern of multiple shoots indicated their origin from an enlarged shoot base via the proliferation of apical shoots. All in vitro regenerated plantlets acclimatized in aquariums successfully. The simplicity of the
